Difference between revisions of "User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/20"

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==Summary==
 
==Summary==
 
* 6% gel HBE en hTERT (AKAP250(AKAP79))
 
* 6% gel HBE en hTERT (AKAP250(AKAP79))
 +
{| border="1"
 +
|+'''6% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]] S0 & C0 are of 7.5.2010'''
 +
! align="center" style="background:#f0f0f0;"|#
 +
! align="center" style="background:#f0f0f0;"|<u>1</u>
 +
! align="center" style="background:#f0f0f0;"|<u>2</u>
 +
! align="center" style="background:#f0f0f0;"|<u>3</u>
 +
! align="center" style="background:#f0f0f0;"|<u>4</u>
 +
! align="center" style="background:#f0f0f0;"|<u>5</u>
 +
! align="center" style="background:#f0f0f0;"|<u>6</u>
 +
! align="center" style="background:#f0f0f0;"|<u>7</u>
 +
! align="center" style="background:#f0f0f0;"|<u>8</u>
 +
! align="center" style="background:#f0f0f0;"|<u>9</u>
 +
! align="center" style="background:#f0f0f0;"|<u>10</u>
 +
! align="center" style="background:#f0f0f0;"|<u>11</u>
 +
! align="center" style="background:#f0f0f0;"|<u>12</u>
 +
! align="center" style="background:#f0f0f0;"|<u>13</u>
 +
! align="center" style="background:#f0f0f0;"|<u>14</u>
 +
! align="center" style="background:#f0f0f0;"|<u>15</u>
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|-
 +
!Gel<br>Lysates
 +
| align="center"|[http://openwetware.org/images/7/74/Spectra_Multicolor_High_Range_Protein_Ladder_-_Fermentas.jpg Fermantas high range Marker]<br> (10 μL)
 +
| align="center"|HeLa -S<br>(40 μL)
 +
| align="center"|Jurkat <br>(40 μL)
 +
| align="center"|HEK293<br>(40 μL)
 +
| align="center"|HBE<br> CTR<br> (20 μL)
 +
| align="center"|HBE<br> 5% CSE (6h)<br> (20 μL)
 +
| align="center"|S0<br>(40 μL)
 +
| align="center"|S1<br>(40 μL)
 +
| align="center"|S2<br>(40 μL)
 +
| align="center"|S3<br>(40 μL)
 +
| align="center"|C0<br>(40 μL)
 +
| align="center"|C1<br>(40 μL)
 +
| align="center"|C2<br>(40 μL)
 +
| align="center"|C3<br>(40 μL) 
 +
| align="center"|X
 +
|-
 +
|}
 +
* 18S qPCR
 +
* 10% repeat yesterday (new ECL)
 
<!--
 
<!--
 
==Materials & Methods==
 
==Materials & Methods==
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===Related topics===
 
===Related topics===
 
*
 
*
 +
-->
 +
 
==Attachment==
 
==Attachment==
 
<html>
 
<html>
<a href="http://openwetware.org/wiki/Image:FILE.xls">
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<a href="http://openwetware.org/wiki/Image:19Oct2010_AKAP9_in_hTERT.xls">
 
   <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">
 
   <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">
 
</a>
 
</a>
</html> [[Media:FILE.xls| Excel TITLE]]
+
</html> [[Media:19Oct2010_AKAP9_in_hTERT.xls|qPCR results AKAP9 (hTERT)]]
-->
+
 
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
|}
 
|}
  
 
__NOTOC__
 
__NOTOC__

Revision as of 00:52, 25 October 2010

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Summary

  • 6% gel HBE en hTERT (AKAP250(AKAP79))
6% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes 21June2010 S0 & C0 are of 7.5.2010
# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Gel
Lysates
Fermantas high range Marker
(10 μL)
HeLa -S
(40 μL)
Jurkat
(40 μL)
HEK293
(40 μL)
HBE
CTR
(20 μL)
HBE
5% CSE (6h)
(20 μL)
S0
(40 μL)
S1
(40 μL)
S2
(40 μL)
S3
(40 μL)
C0
(40 μL)
C1
(40 μL)
C2
(40 μL)
C3
(40 μL)
X
  • 18S qPCR
  • 10% repeat yesterday (new ECL)

Attachment

<html> <a href="http://openwetware.org/wiki/Image:19Oct2010_AKAP9_in_hTERT.xls">

  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">

</a> </html> qPCR results AKAP9 (hTERT)