Difference between revisions of "User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/19"

From OpenWetWare
Jump to: navigation, search
m (Summary)
m (Summary)
Line 16: Line 16:
 
* Pierce for HBE cells (see attachment)
 
* Pierce for HBE cells (see attachment)
 
* Blots of 10% gel
 
* Blots of 10% gel
 +
[[Image:19102010_10%25_gel_hTERT.tif|500px]]
 
<!--
 
<!--
 
==Materials & Methods==
 
==Materials & Methods==

Revision as of 00:21, 25 October 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • qPCR hTERT, HEK and Jurkat for AKAP9
    • 35 reactions (mastermix) (262.5 μL Blue mix, 26.25 μL primer/probe, 201.25 μL H2O)
    • 14 μL each well + 1 μL cDNA
  • Pierce for HBE cells (see attachment)
  • Blots of 10% gel

File:19102010 10% gel hTERT.tif

Attachment

<html> <a href="http://openwetware.org/wiki/Image:19102010_Pierce_Lysates_HBE.xls">

  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">

</a> </html> Pierce resultaten HBE (& Jurkat)