Difference between revisions of "User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/19"

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(Autocreate 2010/10/19 Entry for User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization)
 
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==Summary==
 
==Summary==
* Short summary
+
* qPCR hTERT, HEK and Jurkat for AKAP9
 +
** 35 reactions (mastermix) (262.5 μL Blue mix, 26.25 μL primer/probe, 201.25 μL H<sub>2</sub>O)
 +
** 14 μL each well + 1 μL cDNA
 +
* Pierce for HBE cells (see attachment)
 +
* Blots of 10% gel
 +
[[Image:19102010_10%25_gel_hTERT.tif|500px]]
 
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==Materials & Methods==
 
==Materials & Methods==
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===Related topics===
 
===Related topics===
 
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==Attachment==
 
==Attachment==
 
<html>
 
<html>
<a href="http://openwetware.org/wiki/Image:FILE.xls">
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<a href="http://openwetware.org/wiki/Image:19102010_Pierce_Lysates_HBE.xls">
 
   <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">
 
   <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">
 
</a>
 
</a>
</html> [[Media:FILE.xls| Excel TITLE]]
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</html> [[Media:19102010_Pierce_Lysates_HBE.xls| Pierce resultaten HBE (& Jurkat)]]
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Revision as of 01:21, 25 October 2010

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Summary

  • qPCR hTERT, HEK and Jurkat for AKAP9
    • 35 reactions (mastermix) (262.5 μL Blue mix, 26.25 μL primer/probe, 201.25 μL H2O)
    • 14 μL each well + 1 μL cDNA
  • Pierce for HBE cells (see attachment)
  • Blots of 10% gel

File:19102010 10% gel hTERT.tif

Attachment

<html> <a href="http://openwetware.org/wiki/Image:19102010_Pierce_Lysates_HBE.xls">

  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">

</a> </html> Pierce resultaten HBE (& Jurkat)