Difference between revisions of "User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/18"

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(Autocreate 2010/10/18 Entry for User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization)
 
(Summary)
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==Summary==
 
==Summary==
* Short summary
+
* Loading 10% gel
 +
** Loading volume was corrected according to the quantification of previous blot to obtain equal loading
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|'''S0'''
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| align="center" style="background:#f0f0f0;"|'''S1'''
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| align="center" style="background:#f0f0f0;"|'''S2'''
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| align="center" style="background:#f0f0f0;"|'''S3'''
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| align="center" style="background:#f0f0f0;"|'''C0'''
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| align="center" style="background:#f0f0f0;"|'''C1'''
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| align="center" style="background:#f0f0f0;"|'''C2'''
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| align="center" style="background:#f0f0f0;"|'''C3'''
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|-
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| Loading volume (corrected)||||||||||||||||
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|}
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*
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{| border="1"
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|+'''10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]] S0 & C0 are of 7.5.2010'''
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! align="center" style="background:#f0f0f0;"|#
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! align="center" style="background:#f0f0f0;"|<u>1</u>
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! align="center" style="background:#f0f0f0;"|<u>2</u>
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! align="center" style="background:#f0f0f0;"|<u>3</u>
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! align="center" style="background:#f0f0f0;"|<u>4</u>
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! align="center" style="background:#f0f0f0;"|<u>5</u>
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! align="center" style="background:#f0f0f0;"|<u>6</u>
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! align="center" style="background:#f0f0f0;"|<u>7</u>
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! align="center" style="background:#f0f0f0;"|<u>8</u>
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! align="center" style="background:#f0f0f0;"|<u>9</u>
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! align="center" style="background:#f0f0f0;"|<u>10</u>
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! align="center" style="background:#f0f0f0;"|<u>11</u>
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! align="center" style="background:#f0f0f0;"|<u>12</u>
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! align="center" style="background:#f0f0f0;"|<u>13</u>
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! align="center" style="background:#f0f0f0;"|<u>14</u>
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! align="center" style="background:#f0f0f0;"|<u>15</u>
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|-
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!Gel<br>Lysates
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| align="center"|X
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| align="center"|[http://openwetware.org/images/7/74/Spectra_Multicolor_High_Range_Protein_Ladder_-_Fermentas.jpg Fermantas high range Marker]<br> (5 μL)
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| align="center"|HeLa -S<br>(40 μL)
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| align="center"|Jurkat <br>(40 μL)
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| align="center"|HEK293<br>(40 μL)
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| align="center"|[http://openwetware.org/images/7/74/Spectra_Multicolor_High_Range_Protein_Ladder_-_Fermentas.jpg Fermantas high range Marker]<br> (10 μL)
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| align="center"|S0<br>(10,1 μL)
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| align="center"|S1<br>(27,5 μL)
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| align="center"|S2<br>(10,8 μL)
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| align="center"|S3<br>(17,2 μL)
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| align="center"|C0<br>(40,0 μL)
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| align="center"|C1<br>(13,8 μL)
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| align="center"|C2<br>(38,3 μL)
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| align="center"|C3<br>(17,2 μL) 
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| align="center"|X
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|-
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|}
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* Gel was cut in 4 pieces and treated with either GAPDH (1:2000, mouse), RII (1:500, rabbit), AKAP79 (1:500, mouse) or Gravin (1:500, rabbit)
 
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==Materials & Methods==
 
==Materials & Methods==

Revision as of 04:35, 19 October 2010

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Summary

  • Loading 10% gel
    • Loading volume was corrected according to the quantification of previous blot to obtain equal loading
' S0 S1 S2 S3 C0 C1 C2 C3
Loading volume (corrected)
10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes 21June2010 S0 & C0 are of 7.5.2010
# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Gel
Lysates
X Fermantas high range Marker
(5 μL)
HeLa -S
(40 μL)
Jurkat
(40 μL)
HEK293
(40 μL)
Fermantas high range Marker
(10 μL)
S0
(10,1 μL)
S1
(27,5 μL)
S2
(10,8 μL)
S3
(17,2 μL)
C0
(40,0 μL)
C1
(13,8 μL)
C2
(38,3 μL)
C3
(17,2 μL)
X
  • Gel was cut in 4 pieces and treated with either GAPDH (1:2000, mouse), RII (1:500, rabbit), AKAP79 (1:500, mouse) or Gravin (1:500, rabbit)