Difference between revisions of "User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/12"

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* Membranes were washed 3x 15min. separately depending on secondary antibody
 
* Membranes were washed 3x 15min. separately depending on secondary antibody
 
* α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours
 
* α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours
 +
* Preparation of 2x 10% tris-glycine gels
 
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==Materials & Methods==
 
==Materials & Methods==

Revision as of 02:31, 12 October 2010

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Summary

  • Membranes were washed 3x 15min. separately depending on secondary antibody
  • α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours
  • Preparation of 2x 10% tris-glycine gels