User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/11: Difference between revisions
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* Blocking membrane of [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/10/08|08October2010]] → Incubating membrane with RIIα (sc-909) and Gravin (animal 122)dilution 1:500 ON @ 4°C | * Blocking membrane of [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/10/08|08October2010]] → Incubating membrane with RIIα (sc-909) and Gravin (animal 122)dilution 1:500 ON @ 4°C | ||
* Preparation of 2 10% Tris-glycine acrylamide gels | * Preparation of 2 10% Tris-glycine acrylamide gels | ||
* Dilution of samples, corrected to lowest concentration | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample''' | |||
| align="center" style="background:#f0f0f0;"|''' μL Sample''' | |||
| align="center" style="background:#f0f0f0;"|'''μL dH2O''' | |||
|- | |||
| HEK293 (12/08/2010)||61,5||88,5 | |||
|- | |||
| HeLa -S (p17 (20.6.2010)||26,0||124,0 | |||
|- | |||
| S0 (7.5.2010)||236,9||0,0 | |||
|- | |||
| S1||52,1||97,9 | |||
|- | |||
| S2||150,0||0,0 | |||
|- | |||
| S3||53,8||96,2 | |||
|- | |||
| C0 (7.5.2010)||2470,4||0,0 | |||
|- | |||
| C1||59,6||90,4 | |||
|- | |||
| C2||36,1||113,9 | |||
|- | |||
| C3||106,3||43,7 | |||
|} | |||
{| border="1" | {| border="1" | ||
|+'''10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]] S0 & C0 are of 7.5.2010''' | |+'''10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]] S0 & C0 are of 7.5.2010''' |
Revision as of 05:56, 11 October 2010
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Summary
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