User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/25

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<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Owwnotebook icon.pngRII Overlay all <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • All HEK293 Cells died, realized that it had not been 15% but 45% CSE!! Experiment failed, no repeat due to time issues.
  • Aided Caroline in her Cotransin experiment with the ASMC
  • RII Overlay results
  • Purified Rabbit IgG I5006 Sigma Aldrich

Results

  • RII Overlay

File:25082010 RII Overlay cAMP precip AKAP18δ-L314E.TIF File:25082010 RII Overlay cAMP precip AKAP18δ-PP.TIF File:25082010 RII Overlay cAMP precip.TIF
File:25082010 RII Overlay Lysates AKAP18δ-L314E.TIF File:25082010 RII Overlay Lysates AKAP18δ-PP.TIF File:25082010 RII Overlay Lysates.TIF
File:25082010 RII Overlay PKA Co-IP.TIF

Discussion

  • PKA Co-IP Appears still unsuccessful, at least RII subunits would be expected to be visible
  • Even AKAP18δ-PP seems to inhibit some RII binding, seeing as the signal is somewhat fainter compared to the untreated blots