User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/16

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<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Owwnotebook icon.pngSDS-PAGE lysates, Cell splitting <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • 2x SDS-PAGE
4-12% Gradient NuPage Gel loading LYSATES hTERT D9 + HEK293, HeLa -S & Jurkat see codes 21June2010
# 1 2 3 4 5 6 7 8 9 10
Gel
Lysates
Invitrogen Marker
(8 μL)
Jurkat
(20 μL)
HEK293
(20 μL)
Biorad Marker
(8 μL)
C1
(20 μL)
C2
(20 μL)
C3
(20 μL)
S1
(20 μL)
S2
(20 μL)
S3
(20 μL)
    • NOTE: After 60 min. the SDS-PAGE was put to ice and the voltage was turned from 200 V to 100 V, to latter because of limitation in the amount of people running wet blots.
    • Both gels were blotted using the NuPage wetblot system, 2 h @ 30 V
  • Cells were splitted to D9 P28
  • Cells in 24-wells were put to S0

Results

  • Ponceau staining of blots

File:16082010 Ponceau Lysates Blot -1.TIFFile:Coomassie gel after blot 16.8.10-1.tif
File:16082010 Ponceau Lysates Blot -2.TIFFile:Coomassie gel after blot 16.8.10-2.tif

Discussion

  • DONE Ow..