User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/12

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<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Owwnotebook icon.pngLyse HEK293 (isolate RNA), 2nd AB WB, Convocal microscopy <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • 2nd Antibody (mouse) on WB, 1:1000 dilution (SHOULD BE 1:10.000)
  • RIPA (Lysis) Buffer (per 1 mL)
    • 1.06 mg β-Glycerolphosphate
    • 1 mL RIPA buffer
    • 8 μL Protease inhibitor mix
    • 1 μL NaF (1 M)
    • 10 μL NaVO3 (10 mM)
    • 12.5 μL PMSF
  • Lysate HEK293 Cells
  • CONVOCAL MICROSCOPY!
  • TODO Isolate RNA of HEK293 Cells

Results

  • Western blot

File:10082010 WB.tif

  • Quick preview of IF results
    • No good picture of Gravin localisation.
    • And no changes in RII with Ht31

IF, CTRvsCSE.jpg

Discussion

  • DONE Ow..

Attachment

<html> <a href="http://openwetware.org/wiki/Image:12082010_RNA_concentration_HEK293.pdf">

  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">

</a> </html> RNA concentration HEK293