User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/11

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Owwnotebook icon.pngJurkat qPCR results, Primary antibody WB, Ending stimulation ELISA <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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  • Western blot of yesterday was cut in 3 and incubated with primary antibody
    • AKAP450 (mouse, BD Bioscience, 611518)
    • RIIα(mouse, BD Bioscience, 612243)
    • AKAP95 (mouse, Transduction Laboratories, 610995)
    • As internal control also GAPDH need to be measured,
    • 20 µL reaction volume, as given by the machine
    • Human GAPDH primer (Applied biosystems)
    • Taqman Gene expression master mix


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</a> </html> qPCR Jurkat expression AKAP12, AKAP9, GAPDH

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</a> </html> ELISA VIABILITY STAINING ONLY (Alamar blue)