User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/09

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Owwnotebook icon.pngIsolate Protein/RNA Jurkat cells, Codes IF <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • Isolate proteins and RNA from Jurkat cells (P7)
    • Centrifuge 5 min. 300xg to collect the cells
    • Instead of 0.2 mL/mL TRIzol; 400 μL for the 6 mL TRIzol
    • 2 mL of isopropanol (instead of 0.5 mL/mL TRIzol

Attachment

<html> <a href="http://openwetware.org/wiki/Image:IF_09082010_D9_P29.xls">

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</a> </html> IF D9 P29

<html> <a href="http://openwetware.org/wiki/Image:09082010_jurkat_rnapdf.pdf">

  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">

</a> </html> RNA concentration Jurkat cells