User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/03

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Summary

  • ELISA (Caro (12:08) & Mine (11:55))
    • Mine was the same as the previous (01August2010)
    • NOTE: FORGOT TO ADD DMSO TO THE ONES NOT RECEIVING Ht31!
Caro.100803 ' Control 8Pcpt 6Bnz Ht31 a.o. DMSO Cotransin
1 2 3 4 5 6
CTR A CTR CTR+8P CTR+6Bnz CSE+Ht31 DMSO+CTR Cotransin+CTR (2/400)
CTR B CTR CTR+8P CTR+6Bnz CSE+Ht31 DMSO+CTR Cotransin+CTR (6/400)
CSE C CSE CSE+8P CSE+6Bnz CSE+Ht31+6Bnz DMSO+CSE Cotransin+CSE (2/400)
CSE D CSE CSE+8P CSE+6Bnz CSE+Ht31+6Bnz DMSO+CSE Cotransin+CSE (6/400)
  • New cells were plated out, D9 P23
  • RII Overlay showed NOTHING!! Not even marker signals...
    • Most probable cause, blot was incubated ON up side down?
  • IF Cells seeded in 100.000 cells per plate are swarming the coverslips, only use 75.000 cells from now on