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 ELISA, RII, IF
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Summary
- ELISA (Caro (12:08) & Mine (11:55))
- Mine was the same as the previous (01August2010)
- NOTE: FORGOT TO ADD DMSO TO THE ONES NOT RECEIVING Ht31!
| Caro.100803
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'
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Control
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8Pcpt
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6Bnz
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Ht31 a.o.
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DMSO
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Cotransin
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1 |
2 |
3 |
4 |
5 |
6
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| CTR |
A |
CTR |
CTR+8P |
CTR+6Bnz |
CSE+Ht31 |
DMSO+CTR |
Cotransin+CTR (2/400)
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| CTR |
B |
CTR |
CTR+8P |
CTR+6Bnz |
CSE+Ht31 |
DMSO+CTR |
Cotransin+CTR (6/400)
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| CSE |
C |
CSE |
CSE+8P |
CSE+6Bnz |
CSE+Ht31+6Bnz |
DMSO+CSE |
Cotransin+CSE (2/400)
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| CSE |
D |
CSE |
CSE+8P |
CSE+6Bnz |
CSE+Ht31+6Bnz |
DMSO+CSE |
Cotransin+CSE (6/400)
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- New cells were plated out, D9 P23
- RII Overlay showed NOTHING!! Not even marker signals...
- Most probable cause, blot was incubated ON up side down?
- IF Cells seeded in 100.000 cells per plate are swarming the coverslips, only use 75.000 cells from now on
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ELISA, RII, IF
