User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/29

From OpenWetWare

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Owwnotebook icon.pngPKA-Co-IP gel <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • 4-12% Gradient NuPage Gel
Gel loading hTERT D9 + HEK293 & HeLa -S see codes 21June2010
# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Gel
PKA Co-IP
X X Invitrogen Marker
(8 μL)
HeLa -S
(20 μL)
HEK293
(20 μL)
Biorad Marker
(8 μL)
C1
(20 μL)
C2
(20 μL)
C3
(20 μL)
S1
(20 μL)
S2
(20 μL)
S3
(20 μL)
X X X