User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/28

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Owwnotebook icon.pngImmunofluorescence for cells, Gravin & RII localization <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • Stopped CSE treatment and started immunofluorescence protocol
    • cells: D9 P25
    • Primary antibody both diluted 1:1000 in the same suspension
  • Secondary antibody diluted 1:300 and DAPI staining (50x) diluted 1:50
    • NOTE: They might have been too long in dH2O
  • Finished PKA Co-IP and added 50 μL of NuPage loading buffer + DTT (stored @ -20°C)
  • Split cells D9 P26 to P27