User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/05

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  • Bradford (Enspire 2300 & Tecan Safire)
  • cAMP precipitation


  • Sadly no difference could be seen between Enspire and the Tecan reader, and the RIPA buffer (not lysis), still yielded a very high signal
  • Everything was put equal to sample S1 and 1 mL of put to 30 μL of 8-AHA-cAMP agarose beads (3x)


  • Maybe Pierce assay might be a better alternative to the Bradford assay is something in the buffer is interfering


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</a> </html> Bradford results both Tecan and Enspire