User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/13

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Owwnotebook icon.pngPutting cells to S0, splitting cells and continuing of protein isolation <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • Protein pellets left @ 4 °C have been resuspended while in storage, only minor pellet remains
    • For final resuspending samples were incubated @ 50 °C and vortexed until resuspended
  • Cells of D9 P28 were put to S0
  • Cells were split to D9 P29 (800.000 cells per plate)

Notes

  • Cells were letting go a bit during washing, perhaps too confluent and dying?