User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/02

From OpenWetWare
Jump to: navigation, search

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Owwnotebook icon.pngPreparing TRIS Urea gel <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • 8% AA Tris-Urea gel was prepared (separating)
    • Tris pH 8.8 2.5 mL (Should be 1.5 M? But 0.75 M was used)
    • 4.8 g Urea
    • 2.7 mL 30% bis-Acrylamide
    • 100 μL 10% APS
    • 6 μL TEMED
    • Up to 10 mL with H2O (about 1 mL?)
  • 5% AA Tris-Urea gel was prepared (Stacking)
    • Tris pH 6.8 630 μL (Should be 1 M? But 0.625 M was used)
    • 2.4 g Urea
    • 830 μL 30% bis-Acrylamide
    • 50 μL 10% APS
    • 5 μL TEMED
    • Up to 5 mL with H2O (about 2.5 mL?)

Discussion

  • Does the Tris concentration hurt the gel??