Difference between revisions of "User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization"

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(Table of contents)
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*29/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/29|cAMP precipitation part II & SDS-PAGE]]
 
*29/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/29|cAMP precipitation part II & SDS-PAGE]]
 
*30/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/30|Lyse HeLa]]
 
*30/06/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/30|Lyse HeLa]]
 +
<b>July</b>
 
*01/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/01|Bradford, qPCR AKAP12 & AKAP9, Silverstaining]]
 
*01/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/01|Bradford, qPCR AKAP12 & AKAP9, Silverstaining]]
*02/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/02|xx]]
+
*02/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/02|qPCR GAPDH, Silverstaining]]
*03/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/03|xx]]
+
*03/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/03|Silver staining attempt #3]]
*05/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/05|xx]]
+
*05/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/05|Bradford & cAMP precipitation]]
*06/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/06|xx]]
+
*06/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/06|cAMP precipitation gel]]
*07/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/07|xx]]
+
*07/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/07|RII overlay cAMP precipation]]
*08/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/08|xx]]
+
*08/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/08|RII overlay result]]
*09/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/09|xx]]
+
*09/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/09|Gravin Co-IP, cAMP precipation gel]]
*10/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/10|xx]]
+
*10/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/10|Gravin Co-IP gel]]
*11/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/11|xx]]
+
*11/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/11|AKAP250 (scbt) immunoblot cAMP precipitation]]
*12/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/12|xx]]
+
*12/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/12|AKAP250 (scbt) immunoblot AKAP250 Co-IP]]
*13/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/13|xx]]
+
*{{todo}} 13/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/13|AKAP250 (Eurogentech) immunoblot]]
*14/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/14|xx]]
+
*{{todo}} 14/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/14|AKAP250 (abcam) immunoblot]]
*15/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/15|xx]]
+
*15/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/15|Epac immunoblot]]
*16/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/16|xx]]
+
*{{todo}} 16/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/16|Epac immunoblot result]]
*19/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/19|xx]]
+
*19/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/19|Epac immunoblot repeat, new cell culture]]
*20/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/20|xx]]
+
*{{todo}} 20/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/20|Epac immunoblot repeat result]]
*21/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/21|xx]]
+
*21/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/21|Splitting cells]]
*24/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/24|xx]]
+
*24/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/24|Splitting cells for immunofluorescence]]
*25/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/25|xx]]
+
*25/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/25|Cell check]]
*26/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/26|xx]]
+
*26/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/26|Cover slips to S<sup>0</sup> & Plating out for ELISA]]
*27/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/27|xx]]
+
*27/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/27|Stimulation cover slips, PKA-Co-IP]]
*28/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/28|xx]]
+
*28/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/28|Immunofluorescence for cells, Gravin & RII localization]]
*29/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/29|xx]]
+
*29/07/2010: [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/29|PKA-Co-IP gel]]
 
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Revision as of 05:50, 29 July 2010

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Project Description/Abstract

“Towards insights into cyclic AMP compartmentalization in cell of lung origin” a cooperation between the department of Molecular Pharmacology (University Groningen, the Netherlands) and the Leibniz-Institut für Molekulare Pharmakologie (Berlin, Germany). These are the entries of a Master internship from February 'till September 2010.

Notes

Interesting entries

Protocols

Results

ELISA
  1. Viability & ELISA
  2. Dose effect curve ELISA
  3. ELISA
  4. ELISA
  5. ..
  6. ELISA Berlin Okay
Co-IP
  1. Co-IP 1 Anouk
  2. Co-IP 2 Anouk
  3. Co-IP 3 Anouk
  4. Co-IP 4 TODO
RII Overlay
  1. ..
  2. RII overlay Okay
Misc.

Convenient Links

Downloads

<html> <a href="http://openwetware.org/wiki/Image:BLANCO_ELISA_COPY_ME_BEFORE_USE.xls">

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</a> </html> Blanc for IL-8 ELISA (Linear regression)

<html> <a href="http://openwetware.org/wiki/Image:BLANCO_PROTEIN_SIZE.xls">

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</a> </html> Blanc for protein size on gel/membrane (non-Linear regression)

Table of contents

'
Groningen Berlin

February

March

April

May

June

July

Recent changes

23 September 2017

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     11:33  BIOL398-04/S15:Class Assignments by Week‎ (diff | hist) . . (-3). . Redirect fixer (talk | contribs) (Automatically fixing double redirect from Tessa A. Morris Class Assignments by Week to Tessa Morris Class Assignments by Week in a maintenance job.)
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