Difference between revisions of "User:Wesley J. Houston/Notebook/MAP/2013/03/26"

From OpenWetWare
< User:Wesley J. Houston‎ | Notebook‎ | MAP‎ | 2013‎ | 03
Jump to: navigation, search
(Entry title)
(fix raw html notebook nav)
 
(2 intermediate revisions by one other user not shown)
Line 2: Line 2:
 
|-
 
|-
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
+
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
|-
 
|-
 
| colspan="2"|
 
| colspan="2"|
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==3/26==
+
==3/26/13==
 
* Prepped plasmids of KAH182 and KAH201 liquid cultures, following the Zyppy plasmid miniprep kit.
 
* Prepped plasmids of KAH182 and KAH201 liquid cultures, following the Zyppy plasmid miniprep kit.
 
* Determined yields of plasmids.
 
* Determined yields of plasmids.
 +
  
 
{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table -->
 
{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table -->
Line 18: Line 19:
 
| 2. KAH201 (Plasmid) || 0.069 || 1.857 || 69.164
 
| 2. KAH201 (Plasmid) || 0.069 || 1.857 || 69.164
 
|}
 
|}
 +
  
 
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->
 
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->
Line 37: Line 39:
 
|}
 
|}
  
> Measure conc.'s of gel purified fragments
+
 
 +
> Measure conc.'s of gel purified fragments (KAH182 = lanes 2 and 3; KAH201 = lanes 4 and 5)
 
{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table -->
 
{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table -->
 
|-
 
|-

Latest revision as of 21:35, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

3/26/13

  • Prepped plasmids of KAH182 and KAH201 liquid cultures, following the Zyppy plasmid miniprep kit.
  • Determined yields of plasmids.


Sample OD260 260/280 ng/μL
1. KAH201 (Plasmid) 0.056 1.732 56.395
2. KAH201 (Plasmid) 0.069 1.857 69.164


Reagent Volume   Results of the fifth electrophoresis attempt
30 μL/lane, 1% agarose; Ladder
DNA (plasmid) 25 μL
10x buffer 3.0
enzyme 1 (Pst1) 1.0
enzyme 2 (Xba1 or Spe1) 1.0
dH2O 0.0 μL
  30 μL --> 37°C/ ~15 min.


> Measure conc.'s of gel purified fragments (KAH182 = lanes 2 and 3; KAH201 = lanes 4 and 5)

Sample OD260 260/280 ng/μL
1. KAH201 (S/P) 0.029 1.054 29.262
2. KAH201 (S/P) 0.006 1.381 6.172
3. KAH182 (X/P) -0.002 2 -1.702
4. KAH182 (X/P) 0.007 5.583 7.129