Difference between revisions of "User:Wesley J. Houston/Notebook/MAP/2013/03/25"

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(Autocreate 2013/03/25 Entry for User:Wesley_J._Houston/Notebook/MAP)
 
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==Entry title==
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==3/25/13==
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{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->
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|-valign="top"
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| <u>Reagent</u> || <u>Volume</u> || &nbsp;
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| rowspan="9" | [[Image:Electrophoresis5.jpg‎ |150px|Results of the fourth electrophoresis attempt]]<br>15 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
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|-
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| DNA (plasmid) || 25 μL
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|-
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| 10x buffer || 3.0
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|-
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| enzyme 1 (Pst1) || 1.0
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|-
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| enzyme 2 (Xba1 or Spe1)|| 1.0
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|-
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| dH<sub>2</sub>O || 0.0 μL
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|-
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| &nbsp; || 30 μL --> 37°C/ ~15 min.
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|}
  
 
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Revision as of 15:27, 25 March 2013

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3/25/13

Reagent Volume   Results of the fourth electrophoresis attempt
15 μL/lane, 1% agarose; Ladder
DNA (plasmid) 25 μL
10x buffer 3.0
enzyme 1 (Pst1) 1.0
enzyme 2 (Xba1 or Spe1) 1.0
dH2O 0.0 μL
  30 μL --> 37°C/ ~15 min.