User:Wesley J. Houston/Notebook/MAP/2013/03/22: Difference between revisions

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|-valign="top"
|-valign="top"
| <u>Reagent</u> || <u>Volume</u> || &nbsp;
| <u>Reagent</u> || <u>Volume</u> || &nbsp;
| rowspan="9" | [[Image:Electrophoresis4.jpg‎ |150px|Results of third electrophoresis]]<br>15 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
| rowspan="9" | [[Image:Electrophoresis4.jpg‎ |150px|Results of third electrophoresis]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
|-
|-
| DNA (plasmid) || 20 μL
| DNA (plasmid) || 20 μL

Revision as of 22:05, 25 March 2013

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3/22/13

Reagent Volume   Results of third electrophoresis
30 μL/lane, 1% agarose; Ladder
DNA (plasmid) 20 μL
10x buffer 3.0
enzyme 1 (Pst1) 1.0
enzyme 2 (Xba1 or Spe1) 1.0
dH2O 5.0 μL
  30 μL --> 37°C/ ~15 min.

> Measure conc.'s of gel purified fragments

Sample 260/280 ng/μL
1. KAH201 (S/P) 0.32 0.851
2. KAH182 (X/P) 0.385 1.064



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