Difference between revisions of "User:Wesley J. Houston/Notebook/MAP/2013/03/22"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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* Gel purification was performed using the KAH201 plasmids prepped on 2/28, as well as the KAH182 plasmids prepped on 3/21.
 
* Gel purification was performed using the KAH201 plasmids prepped on 2/28, as well as the KAH182 plasmids prepped on 3/21.
 
** No DNA was present in the second lane, and the concentrations and purities were determined to be too low to complete a ligation.
 
** No DNA was present in the second lane, and the concentrations and purities were determined to be too low to complete a ligation.
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* Grew more KAH201 liquid cultures.
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** Placed colonies in LB broth overnight in shaking 37C incubator.
  
 
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->
 
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->

Latest revision as of 22:34, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

3/22/13

  • Gel purification was performed using the KAH201 plasmids prepped on 2/28, as well as the KAH182 plasmids prepped on 3/21.
    • No DNA was present in the second lane, and the concentrations and purities were determined to be too low to complete a ligation.
  • Grew more KAH201 liquid cultures.
    • Placed colonies in LB broth overnight in shaking 37C incubator.
Reagent Volume   Results of third electrophoresis
30 μL/lane, 1% agarose; Ladder
DNA (plasmid) 20 μL
10x buffer 3.0
enzyme 1 (Pst1) 1.0
enzyme 2 (Xba1 or Spe1) 1.0
dH2O 5.0 μL
  30 μL --> 37°C/ ~15 min.

> Measure conc.'s of gel purified fragments

Sample 260/280 ng/μL
1. KAH201 (S/P) 0.32 0.851
2. KAH182 (X/P) 0.385 1.064