Difference between revisions of "User:Wesley J. Houston/Notebook/MAP/2013/03/21"

From OpenWetWare
< User:Wesley J. Houston‎ | Notebook‎ | MAP‎ | 2013‎ | 03
Jump to: navigation, search
(Entry title)
(3/21/13)
Line 8: Line 8:
 
==3/21/13==
 
==3/21/13==
 
* Retrieved liquid cultures from the incubator.
 
* Retrieved liquid cultures from the incubator.
* Pelleted the samples (3 mins @ 16.3k RPM).
+
* Pelleted the samples (3 mins @ 16.3k x g).
 
** No pellets of KAH201 were produced.
 
** No pellets of KAH201 were produced.
 
** KAH182 was successfully pelleted.
 
** KAH182 was successfully pelleted.

Revision as of 14:24, 25 March 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

3/21/13

  • Retrieved liquid cultures from the incubator.
  • Pelleted the samples (3 mins @ 16.3k x g).
    • No pellets of KAH201 were produced.
    • KAH182 was successfully pelleted.
  • Next, I followed the established protocol for the Zyppy plasmid prep kit and made plasmids of KAH182.
    • Concentration and purity was also determined (43.201, 1.758 (1); 40.434, 1.751(2)).
      • Note there were two samples of KAH182.
  • Since the previously made KAH201 colonies (made on Jan 24, 2013) appear to not be viable, I have also made more KAH201 colonies.
    • Following the established protocol for bacterial transformation.