Difference between revisions of "User:Wesley J. Houston/Notebook/MAP/2013/03/21"

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(3/21/13)
(3/21/13)
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** KAH182 was successfully pelleted.
 
** KAH182 was successfully pelleted.
 
* Next, I followed the established protocol for the Zyppy plasmid prep kit and made plasmids of KAH182.
 
* Next, I followed the established protocol for the Zyppy plasmid prep kit and made plasmids of KAH182.
** Concentration and purity was also determined (43.201, 1.758 (1); 40.434, 1.751(2)).
+
** Concentration and purity was also determined.
 
*** Note there were two samples of KAH182.
 
*** Note there were two samples of KAH182.
 
* Since the previously made KAH201 colonies (made on Jan 24, 2013) appear to not be viable, I have also made more KAH201 colonies.
 
* Since the previously made KAH201 colonies (made on Jan 24, 2013) appear to not be viable, I have also made more KAH201 colonies.
 
** Following the established protocol for bacterial transformation.
 
** Following the established protocol for bacterial transformation.
  
 +
{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table -->
 +
|-
 +
| <u>Sample</u> || <u>260/280</u> || <u>ng/μL</u>
 +
|-
 +
| 1. KAH182 (Plasmid) || 1.758 || 43.201
 +
|-
 +
| 2. KAH182 (Plasmid) || 7.751 || 40.434
 +
|}
  
 
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Revision as of 21:16, 25 March 2013

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3/21/13

  • Retrieved liquid cultures from the incubator.
  • Pelleted the samples (3 mins @ 16.3k x g).
    • No pellets of KAH201 were produced.
    • KAH182 was successfully pelleted.
  • Next, I followed the established protocol for the Zyppy plasmid prep kit and made plasmids of KAH182.
    • Concentration and purity was also determined.
      • Note there were two samples of KAH182.
  • Since the previously made KAH201 colonies (made on Jan 24, 2013) appear to not be viable, I have also made more KAH201 colonies.
    • Following the established protocol for bacterial transformation.
Sample 260/280 ng/μL
1. KAH182 (Plasmid) 1.758 43.201
2. KAH182 (Plasmid) 7.751 40.434