Difference between revisions of "User:Wesley J. Houston/Notebook/MAP/2013/03/21"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==3/21/13==
 
==3/21/13==
 
* Retrieved liquid cultures from the incubator.
 
* Retrieved liquid cultures from the incubator.
* Pelleted the samples (3 mins @ 16.3k RPM).
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* Pelleted the samples (3 mins @ 16.3k x g).
 
** No pellets of KAH201 were produced.
 
** No pellets of KAH201 were produced.
 
** KAH182 was successfully pelleted.
 
** KAH182 was successfully pelleted.
 
* Next, I followed the established protocol for the Zyppy plasmid prep kit and made plasmids of KAH182.
 
* Next, I followed the established protocol for the Zyppy plasmid prep kit and made plasmids of KAH182.
** Concentration and purity was also determined (43.201, 1.758 (1); 40.434, 1.751(2)).
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** Concentration and purity was also determined.
 
*** Note there were two samples of KAH182.
 
*** Note there were two samples of KAH182.
 
* Since the previously made KAH201 colonies (made on Jan 24, 2013) appear to not be viable, I have also made more KAH201 colonies.
 
* Since the previously made KAH201 colonies (made on Jan 24, 2013) appear to not be viable, I have also made more KAH201 colonies.
 
** Following the established protocol for bacterial transformation.
 
** Following the established protocol for bacterial transformation.
  
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{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table -->
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|-
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| <u>Sample</u> || <u>260/280</u> || <u>ng/μL</u>
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|-
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| 1. KAH182 (Plasmid) || 1.758 || 43.201
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|-
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| 2. KAH182 (Plasmid) || 7.751 || 40.434
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|}
  
 
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Latest revision as of 21:34, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

3/21/13

  • Retrieved liquid cultures from the incubator.
  • Pelleted the samples (3 mins @ 16.3k x g).
    • No pellets of KAH201 were produced.
    • KAH182 was successfully pelleted.
  • Next, I followed the established protocol for the Zyppy plasmid prep kit and made plasmids of KAH182.
    • Concentration and purity was also determined.
      • Note there were two samples of KAH182.
  • Since the previously made KAH201 colonies (made on Jan 24, 2013) appear to not be viable, I have also made more KAH201 colonies.
    • Following the established protocol for bacterial transformation.
Sample 260/280 ng/μL
1. KAH182 (Plasmid) 1.758 43.201
2. KAH182 (Plasmid) 7.751 40.434