User:Wesley J. Houston/Notebook/MAP/2013/03/13: Difference between revisions
From OpenWetWare
(2 intermediate revisions by the same user not shown) | |||
Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==3/13/13== | ==3/13/13== | ||
* | * Attempted ligation following the same protocol outlined on 3/4/13. | ||
* | * Grew four liquid cultures of KAH182, following the same procedure (pick a colony and place into LB broth), in case this ligation attempt fails. | ||
> Ligations | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Ligation rxn table --> | |||
| || 1 || 2 || | |||
|- | |||
| Insert DNA (KAH182) || 3.0 || --- || | |||
|- | |||
| Vector DNA (KAH201) || 0.5 || 0.5 || | |||
|- | |||
| 2x lgn buf (Roche) || 5.0 || 5.0 || | |||
|- | |||
| T4 ligase (NEB) || 1.0 || 1.0 || | |||
|- | |||
| dH<sub>2</sub>O || 0.5 || 3.5 || | |||
|- | |||
| || 10 μL || 10 μL || | |||
|} | |||
* Follow quick transformation protocol | |||
* Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar | |||
> Ligations (corrected version, 3/04/13) | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Ligations table --> | |||
| <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> 03/13/13</font> | |||
|- | |||
| 1. KAH201 (S/P)/<font color="blue">3403</font>, 25 ng + KAH182 (X/P)/1675, 26 ng || <font color="blue">0</font> | |||
|- | |||
| 2. KAH201 (S/P)/3403, 25 ng || 0 | |||
|} | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 22:12, 25 March 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||
3/13/13
> Ligations
> Ligations (corrected version, 3/04/13)
|