User:Wesley J. Houston/Notebook/MAP/2013/03/11: Difference between revisions

From OpenWetWare
< User:Wesley J. Houston‎ | Notebook‎ | MAP‎ | 2013‎ | 03
Jump to navigationJump to search
Line 8: Line 8:
==3/11/13==
==3/11/13==
* Grow liquid cultures of KAH182 to repeat ligation.
* Grow liquid cultures of KAH182 to repeat ligation.
* Retry ligation with lower concentration plasmids (will make table).
* Retry ligation with lower concentration plasmids.
 
{| class="wikitable" border="0" cellspacing="3" <!-- Ligation rxn table -->
| &nbsp;            || 1    || 2    ||
|-
| Insert DNA (KAH182)        || 4.0  || ---  ||
|-
| Vector DNA (KAH201)        || 0.65  || 0.65  ||
|-
| 2x lgn buf (Roche) || 5.0  || 5.0  ||
|-
| T4 ligase (NEB)   || 1.0  || 1.0  ||
|-
| dH<sub>2</sub>O    ||  0 ||  3.5 ||
|-
| &nbsp;            || 10 μL || 10 μL ||
|}
 
* Follow quick transformation protocol
* Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar
 




Revision as of 13:12, 18 March 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

3/11/13

  • Grow liquid cultures of KAH182 to repeat ligation.
  • Retry ligation with lower concentration plasmids.
  1 2
Insert DNA (KAH182) 4.0 ---
Vector DNA (KAH201) 0.65 0.65
2x lgn buf (Roche) 5.0 5.0
T4 ligase (NEB) 1.0 1.0
dH2O 0 3.5
  10 μL 10 μL
  • Follow quick transformation protocol
  • Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Wesley_J._Houston/Notebook/MAP/2013/03/11&oldid=683816"