User:Wesley J. Houston/Notebook/MAP/2013/03/08

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3/8/13

  • Ligation was repeated.
  • Attempted to grow more liquid cultures of ligated plasmids.
  • Attempted to confirm ligation by streaking the colonies on an agar plate.
  • All attempts proved unsuccessful.
Reagent Volume   Results of the ligation electrophoresis
15 μL/lane, 1% agarose; Ladder
DNA (plasmid) 20 μL
10x buffer 3.0
Enzyme 1 (Pst1) 1.0
Enzyme 2 (Spe1 or Xba1) 1.0
dH2O 5.0 μL
  30 μL --> 37°C/ ~15 min.