User:Wesley J. Houston/Notebook/MAP/2013/03/07: Difference between revisions

From OpenWetWare
< User:Wesley J. Houston‎ | Notebook‎ | MAP‎ | 2013‎ | 03
Jump to navigationJump to search
Line 20: Line 20:
| 10x buffer || 3.0
| 10x buffer || 3.0
|-
|-
| enzyme 1 || 1.0
| EcoR1 || 1.0
|-
|-
| enzyme 2 || 1.0
| Pst1 || 1.0
|-
|-
| dH<sub>2</sub>O || 5.0 μL
| dH<sub>2</sub>O || 5.0 μL
Line 31: Line 31:


* No DNA present, will repeat ligation.
* No DNA present, will repeat ligation.
** Determined to be a false positive.


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 22:01, 25 March 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

3/7/13

  • Prepped plasmids using Zyppy miniprep kit.
  • Digested plasmids with EcoR1 and Pst1.
  • Ran gel electrophoresis to confirm assembly.
Reagent Volume   Results of the ligation electrophoresis
15 μL/lane, 1% agarose; Ladder
DNA (plasmid) 20 μL
10x buffer 3.0
EcoR1 1.0
Pst1 1.0
dH2O 5.0 μL
  30 μL --> 37°C/ ~15 min.


  • No DNA present, will repeat ligation.
    • Determined to be a false positive.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Wesley_J._Houston/Notebook/MAP/2013/03/07&oldid=685871"