Difference between revisions of "User:Wesley J. Houston/Notebook/MAP/2013/03/07"

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(Autocreate 2013/03/07 Entry for User:Wesley_J._Houston/Notebook/MAP)
 
(3/7/13)
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==Entry title==
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==3/7/13==
* Insert content here...
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* Prepped plasmids using Zyppy miniprep kit.
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* Digested plasmids with EcoR1 and Pst1.
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* Ran gel electrophoresis to confirm assembly.
  
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{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->
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|-valign="top"
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| <u>Reagent</u> || <u>Volume</u> || &nbsp;
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| rowspan="9" | [[Image:Electrophoresis3.jpg‎ |150px|Results of the ligation electrophoresis]]<br>15 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
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|-
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| DNA (plasmid) || 20 μL
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|-
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| 10x buffer || 3.0
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|-
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| EcoR1 || 1.0
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|-
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| Pst1 || 1.0
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|-
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| dH<sub>2</sub>O || 5.0 μL
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|-
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| &nbsp; || 30 μL --> 37°C/ ~15 min.
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|}
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* No DNA present, will repeat ligation.
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** Determined to be a false positive.
  
 
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Revision as of 21:01, 25 March 2013

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3/7/13

  • Prepped plasmids using Zyppy miniprep kit.
  • Digested plasmids with EcoR1 and Pst1.
  • Ran gel electrophoresis to confirm assembly.
Reagent Volume   Results of the ligation electrophoresis
15 μL/lane, 1% agarose; Ladder
DNA (plasmid) 20 μL
10x buffer 3.0
EcoR1 1.0
Pst1 1.0
dH2O 5.0 μL
  30 μL --> 37°C/ ~15 min.


  • No DNA present, will repeat ligation.
    • Determined to be a false positive.