Difference between revisions of "User:Wesley J. Houston/Notebook/MAP/2013/03/07"

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< User:Wesley J. Houston‎ | Notebook‎ | MAP‎ | 2013‎ | 03
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==3/7/13==
 
==3/7/13==
 
* Prepped plasmids using Zyppy miniprep kit.
 
* Prepped plasmids using Zyppy miniprep kit.
* Digested plasmids with EcoR1 and Pst1 (table will be made).
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* Digested plasmids with EcoR1 and Pst1.
* Ran gel electrophoresis to confirm assembly (picture will be uploaded).
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* Ran gel electrophoresis to confirm assembly.
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{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table -->
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|-valign="top"
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| <u>Reagent</u> || <u>Volume</u> || &nbsp;
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| rowspan="9" | [[Image:Electrophoresis3.jpg‎ |150px|Results of the ligation electrophoresis]]<br>15 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
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|-
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| DNA (plasmid) || 20 μL
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|-
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| 10x buffer || 3.0
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|-
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| EcoR1 || 1.0
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|-
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| Pst1 || 1.0
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|-
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| dH<sub>2</sub>O || 5.0 μL
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|-
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| &nbsp; || 30 μL --> 37°C/ ~15 min.
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|}
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* No DNA present, will repeat ligation.
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** Determined to be a false positive.
  
 
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Latest revision as of 22:31, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

3/7/13

  • Prepped plasmids using Zyppy miniprep kit.
  • Digested plasmids with EcoR1 and Pst1.
  • Ran gel electrophoresis to confirm assembly.
Reagent Volume   Results of the ligation electrophoresis
15 μL/lane, 1% agarose; Ladder
DNA (plasmid) 20 μL
10x buffer 3.0
EcoR1 1.0
Pst1 1.0
dH2O 5.0 μL
  30 μL --> 37°C/ ~15 min.


  • No DNA present, will repeat ligation.
    • Determined to be a false positive.