User:Wesley J. Houston/Notebook/MAP/2013/02/08: Difference between revisions
From OpenWetWare
(11 intermediate revisions by the same user not shown) | |||
Line 12: | Line 12: | ||
---- | ---- | ||
'''Assemblies''' | '''Assemblies''' | ||
# KAH201/KAH182: (1) KAH201/( | # KAH201/KAH182: (1) KAH201/(S/P)/203 + (2) KAH182/(X/P)/1675 | ||
> Digests (Fermentas FD)<br> | > Digests (Fermentas FD)<br> | ||
Line 20: | Line 20: | ||
|-valign="top" | |-valign="top" | ||
| <u>Reagent</u> || <u>Volume</u> || | | <u>Reagent</u> || <u>Volume</u> || | ||
| rowspan="9" | [[Image:KAH113012_gel1.jpg |150px| | | rowspan="9" | [[Image:KAH113012_gel1.jpg |150px|Placeholder Image]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| DNA (plasmid) || | | DNA (plasmid) || 20 μL | ||
|- | |- | ||
| 10x buffer || 3.0 | | 10x buffer || 3.0 | ||
Line 30: | Line 30: | ||
| enzyme 2 || 1.0 | | enzyme 2 || 1.0 | ||
|- | |- | ||
| dH<sub>2</sub>O || 0 | | dH<sub>2</sub>O || 5.0 μL | ||
|- | |- | ||
| || 30 μL --> 37°C/ ~15 min. | | || 30 μL --> 37°C/ ~15 min. | ||
Line 41: | Line 41: | ||
| <u>Sample</u> || <u>OD260</u> || <u>260/280</u> || <u>ng/μL</u> | | <u>Sample</u> || <u>OD260</u> || <u>260/280</u> || <u>ng/μL</u> | ||
|- | |- | ||
| 1. KAH201 ( | | 1. KAH201 (S/P) || 0.048 || 1.88 || 48.1 | ||
|- | |- | ||
| 2. KAH182 ( | | 2. KAH182 (X/P) || 0.008 || 1.93 || 8.1 | ||
|} | |} | ||
> Ligations | > Ligations | ||
{| class="wikitable" border="0" cellspacing="3" <!-- Ligations table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Ligations table --> | ||
| <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> mm/dd/yy</font> | | <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> mm/dd/yy</font> | ||
|- | |- | ||
| 1. KAH201 ( | | 1. KAH201 (S/P)/203, 25 ng + KAH182 (X/P)/1675, 6 ng || <font color="blue">TBD</font> | ||
|- | |- | ||
| 2. KAH182 ( | | 2. KAH182 (X/P)/1675 6 ng || TBD | ||
|} | |} | ||
Line 59: | Line 59: | ||
| || 1 || 2 || | | || 1 || 2 || | ||
|- | |- | ||
| Insert DNA || | | Insert DNA || 0.74 || --- || | ||
|- | |- | ||
| Vector DNA || | | Vector DNA || 0.52 || 0.52 || | ||
|- | |- | ||
| 2x lgn buf (Roche) || | | 2x lgn buf (Roche) || 5.0 || 5.0 || | ||
|- | |- | ||
| T4 ligase (NEB) || | | T4 ligase (NEB) || 1.0 || 1.0 || | ||
|- | |- | ||
| dH<sub>2</sub>O || | | dH<sub>2</sub>O || 2.74 || 3.48 || | ||
|- | |- | ||
| || 10 μL || 10 μL || | | || 10 μL || 10 μL || |
Revision as of 13:59, 21 February 2013
Building a Reporter Gene | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
February 8, 2013
Assemblies
> Digests (Fermentas FD)
|