Difference between revisions of "User:Trisha I. Ibeh/Notebook/Trisha Notebook/2014/03/18"

From OpenWetWare
Jump to: navigation, search
(Sample Prep for Stock Solutions)
(Preparation of Magnetic Nanoparticles)
Line 57: Line 57:
 
#Prepare gold: protein mixture with the same ratios as the magnetite nanoparticles  
 
#Prepare gold: protein mixture with the same ratios as the magnetite nanoparticles  
 
#After the samples are in the oven for 2 hours, add the gold:protein mixture to the samples and place in oven for another 2 hours at 80 degrees Celsius
 
#After the samples are in the oven for 2 hours, add the gold:protein mixture to the samples and place in oven for another 2 hours at 80 degrees Celsius
 +
 +
==Atomic Absorption Prep==
 +
 +
Table 1: Prep of Standard Iron Samples
 +
 +
[[Image: Screen_Shot_2014-05-07_at_6.30.22_AM.png ]]
 +
 +
Table 2: Calibration Curve Data
 +
 +
[[Image: Screen_Shot_2014-05-07_at_6.30.12_AM.png]]
 +
 +
Table 3: Atomic Absorption Data
 +
 +
[[Image: Screen_Shot_2014-05-07_at_6.30.28_AM.png ]]
 +
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
|}
 
|}
  
 
__NOTOC__
 
__NOTOC__

Revision as of 03:34, 7 May 2014

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

  1. Run AA on all samples prepared
  2. Preparation of magnetic, gold nanoparticles using different protocol


Sample Prep for Stock Solutions

Table 1: BSA Stock Prep

Screen Shot 2014-05-07 at 4.12.08 AM.png

Table 2: FeSO4 Stock Prep

Screen Shot 2014-05-07 at 4.16.17 AM.png

Table 3: Hemoglobin Prep

Screen Shot 2014-05-07 at 4.12.28 AM.png

Table 4: KNO3 Prep

Screen Shot 2014-05-07 at 4.12.36 AM.png

Table 5: KOH Prep

Screen Shot 2014-05-07 at 4.12.43 AM.png

Table 6: Gold Prep

Screen Shot 2014-05-07 at 4.12.51 AM.png

Table 7: Mag: BSA Prep

Screen Shot 2014-05-07 at 6.24.36 AM.png

Table 8: Mag: Hemoglobin Prep

Screen Shot 2014-05-07 at 6.21.44 AM.png

Table 9: Gold: BSA Prep

Screen Shot 2014-05-07 at 6.27.17 AM.png

Table 10: Gold: Hemoglobin Prep

Screen Shot 2014-05-07 at 6.22.10 AM.png

Preparation of Magnetic Nanoparticles

  1. Prepare 5 mL reaction of magnetic nanoparticles previously used in a 20 mL test tube
  2. Cover test tube with aluminum foil and place in oven for 20 hours at 80 degrees Celsius
  3. Prepare gold: protein mixture with the same ratios as the magnetite nanoparticles
  4. After the samples are in the oven for 2 hours, add the gold:protein mixture to the samples and place in oven for another 2 hours at 80 degrees Celsius

Atomic Absorption Prep

Table 1: Prep of Standard Iron Samples

Screen Shot 2014-05-07 at 6.30.22 AM.png

Table 2: Calibration Curve Data

Screen Shot 2014-05-07 at 6.30.12 AM.png

Table 3: Atomic Absorption Data

Screen Shot 2014-05-07 at 6.30.28 AM.png