Difference between revisions of "User:Trisha I. Ibeh/Notebook/Trisha Notebook/2014/03/18"

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(Entry title)
(Sample Prep for Stock Solutions)
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Table 7: Mag: BSA Prep
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[[Image: Screen_Shot_2014-05-07_at_6.21.44_AM.png]]
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Table 8: Mag: Hemoglobin Prep
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[[Image: Screen_Shot_2014-05-07_at_6.21.44_AM.png ]]
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==Preparation of Magnetic Nanoparticles==
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#Prepare 5 mL reaction of magnetic nanoparticles previously used in a 20 mL test tube
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#Cover test tube with aluminum foil and place in oven for 20 hours at 80 degrees Celsius
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#Prepare gold: protein mixture with the same ratios as the magnetite nanoparticles
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#After the samples are in the oven for 2 hours, add the gold:protein mixture to the samples and place in oven for another 2 hours at 80 degrees Celsius
 
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Revision as of 02:24, 7 May 2014

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Objective

  1. Run AA on all samples prepared
  2. Preparation of magnetic, gold nanoparticles using different protocol


Sample Prep for Stock Solutions

Table 1: BSA Stock Prep

Screen Shot 2014-05-07 at 4.12.08 AM.png

Table 2: FeSO4 Stock Prep

Screen Shot 2014-05-07 at 4.16.17 AM.png

Table 3: Hemoglobin Prep

Screen Shot 2014-05-07 at 4.12.28 AM.png

Table 4: KNO3 Prep

Screen Shot 2014-05-07 at 4.12.36 AM.png

Table 5: KOH Prep

Screen Shot 2014-05-07 at 4.12.43 AM.png

Table 6: Gold Prep

Screen Shot 2014-05-07 at 4.12.51 AM.png

Table 7: Mag: BSA Prep

Screen Shot 2014-05-07 at 6.21.44 AM.png

Table 8: Mag: Hemoglobin Prep

Screen Shot 2014-05-07 at 6.21.44 AM.png

Preparation of Magnetic Nanoparticles

  1. Prepare 5 mL reaction of magnetic nanoparticles previously used in a 20 mL test tube
  2. Cover test tube with aluminum foil and place in oven for 20 hours at 80 degrees Celsius
  3. Prepare gold: protein mixture with the same ratios as the magnetite nanoparticles
  4. After the samples are in the oven for 2 hours, add the gold:protein mixture to the samples and place in oven for another 2 hours at 80 degrees Celsius