User:Trisha I. Ibeh/Notebook/Trisha Notebook/2014/03/18: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Entry title==
==Objective==
* Insert content here...
#Run AA on all samples prepared
#Preparation of magnetic, gold nanoparticles using different protocol


==Sample Prep for Stock Solutions==
Table 1: BSA Stock Prep
[[Image: Screen_Shot_2014-05-07_at_4.12.08_AM.png]] 
Table 2: FeSO4 Stock Prep
[[Image: Screen_Shot_2014-05-07_at_4.16.17_AM.png]]
Table 3: Hemoglobin Prep
[[Image:Screen Shot 2014-05-07 at 4.12.28 AM.png ]]
Table 4: KNO3 Prep
[[Image: Screen_Shot_2014-05-07_at_4.12.36_AM.png ]]
Table 5: KOH Prep
[[Image: Screen_Shot_2014-05-07_at_4.12.43_AM.png]]
Table 6: Gold Prep
[[Image:  Screen_Shot_2014-05-07_at_4.12.51_AM.png]]
Table 7: Mag: BSA Prep
[[Image: Screen_Shot_2014-05-07_at_6.24.36_AM.png]]
Table 8: Mag: Hemoglobin Prep
[[Image: Screen_Shot_2014-05-07_at_6.21.44_AM.png ]]
Table 9: Gold: BSA Prep
[[Image: Screen_Shot_2014-05-07_at_6.27.17_AM.png ]]
Table 10: Gold: Hemoglobin Prep
[[Image: Screen_Shot_2014-05-07_at_6.22.10_AM.png]]
==Preparation of Magnetic Nanoparticles==
#Prepare 5 mL reaction of magnetic nanoparticles previously used in a 20 mL test tube
#Cover test tube with aluminum foil and place in oven for 20 hours at 80 degrees Celsius
#Prepare gold: protein mixture with the same ratios as the magnetite nanoparticles
#After the samples are in the oven for 2 hours, add the gold:protein mixture to the samples and place in oven for another 2 hours at 80 degrees Celsius
==Atomic Absorption Prep==
Table 1: Prep of Standard Iron Samples
[[Image: Screen_Shot_2014-05-07_at_6.30.22_AM.png ]]
Table 2: Calibration Curve Data
[[Image: Screen_Shot_2014-05-07_at_6.30.12_AM.png]]
Table 3: Atomic Absorption Data
[[Image: Screen_Shot_2014-05-07_at_6.30.28_AM.png ]]


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Latest revision as of 23:57, 26 September 2017

Project name Main project page
Previous entry      Next entry

Objective

  1. Run AA on all samples prepared
  2. Preparation of magnetic, gold nanoparticles using different protocol


Sample Prep for Stock Solutions

Table 1: BSA Stock Prep

Table 2: FeSO4 Stock Prep

Table 3: Hemoglobin Prep

Table 4: KNO3 Prep

Table 5: KOH Prep

Table 6: Gold Prep

Table 7: Mag: BSA Prep

Table 8: Mag: Hemoglobin Prep

Table 9: Gold: BSA Prep

Table 10: Gold: Hemoglobin Prep

Preparation of Magnetic Nanoparticles

  1. Prepare 5 mL reaction of magnetic nanoparticles previously used in a 20 mL test tube
  2. Cover test tube with aluminum foil and place in oven for 20 hours at 80 degrees Celsius
  3. Prepare gold: protein mixture with the same ratios as the magnetite nanoparticles
  4. After the samples are in the oven for 2 hours, add the gold:protein mixture to the samples and place in oven for another 2 hours at 80 degrees Celsius

Atomic Absorption Prep

Table 1: Prep of Standard Iron Samples

Table 2: Calibration Curve Data

Table 3: Atomic Absorption Data


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