Difference between revisions of "User:Trisha I. Ibeh/Notebook/Trisha Notebook/2014/01/29"

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(Entry title)
(Magnetic Nanoparticles Day 6)
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*UV Vis was performed on the samples made in the previous lab
 
*UV Vis was performed on the samples made in the previous lab
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Create magnetic BSA-iron NPS
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# Synthesize BSA- iron NPs into test tube that will contain a total of 5mL
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#      BSA- iron solution that has final ratio of 130:1
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# Add 30 mM FeSO4*7H2O, 400 mM KOH, and 100 mM KNO3
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# Water up to 5mL
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# De-gas 130 ratio of hemoglobin- iron solution with nitrogen for 4 hrs at 90 degrees Celsius
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Separately make Hemoglobin- AuNPs the same way as in steps 1-3 but instead of using nitrogen, put test tubes in incubator for 4 hrs at 80 degrees Celsius with ratios of:
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125:1
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130:1
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135:1
  
 
==Notes==
 
==Notes==

Revision as of 12:07, 30 January 2014

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Magnetic Nanoparticles Day 6

  • Mag BSA-AuNPs were created and bubbled through with nitrogen
  • UV Vis was performed on the samples made in the previous lab

Create magnetic BSA-iron NPS

  1. Synthesize BSA- iron NPs into test tube that will contain a total of 5mL
  2. BSA- iron solution that has final ratio of 130:1
  3. Add 30 mM FeSO4*7H2O, 400 mM KOH, and 100 mM KNO3
  4. Water up to 5mL
  5. De-gas 130 ratio of hemoglobin- iron solution with nitrogen for 4 hrs at 90 degrees Celsius



Separately make Hemoglobin- AuNPs the same way as in steps 1-3 but instead of using nitrogen, put test tubes in incubator for 4 hrs at 80 degrees Celsius with ratios of: 125:1 130:1 135:1

Notes

Hemoglobin could not dissolve in water therefore PIs continued with BSA