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==Entry title==
+
==Objective==
* Insert content here...
+
 
 +
==Procedure==
 +
 
 +
==Magnetic Nanoparticles Day 6==
 +
*Mag BSA-AuNPs were created and bubbled through with nitrogen
 +
 
 +
*UV Vis was performed on the samples made in the previous lab
 +
 
 +
Create magnetic BSA-iron NPS
 +
 
 +
# Synthesize BSA- iron NPs into test tube that will contain a total of 5mL
 +
#      BSA- iron solution that has final ratio of 130:1
 +
# Add 30 mM FeSO4*7H2O, 400 mM KOH, and 100 mM KNO3
 +
# Water up to 5mL
 +
# De-gas 130 ratio of hemoglobin- iron solution with nitrogen for 4 hrs at 90 degrees Celsius
 +
 
 +
 
 +
 
 +
 
 +
Separately make Hemoglobin- AuNPs the same way as in steps 1-3 but instead of using nitrogen, put test tubes in incubator for 4 hrs at 80 degrees Celsius with ratios of:
 +
#125:1
 +
#130:1
 +
#135:1
 +
 
 +
==Notes==
 +
Hemoglobin could not dissolve in water therefore PIs continued with BSA
 +
 
 +
 
 +
 
  
  

Revision as of 23:14, 30 April 2014

BDLlogo notext lr.png Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

Procedure

Magnetic Nanoparticles Day 6

  • Mag BSA-AuNPs were created and bubbled through with nitrogen
  • UV Vis was performed on the samples made in the previous lab

Create magnetic BSA-iron NPS

  1. Synthesize BSA- iron NPs into test tube that will contain a total of 5mL
  2. BSA- iron solution that has final ratio of 130:1
  3. Add 30 mM FeSO4*7H2O, 400 mM KOH, and 100 mM KNO3
  4. Water up to 5mL
  5. De-gas 130 ratio of hemoglobin- iron solution with nitrogen for 4 hrs at 90 degrees Celsius



Separately make Hemoglobin- AuNPs the same way as in steps 1-3 but instead of using nitrogen, put test tubes in incubator for 4 hrs at 80 degrees Celsius with ratios of:

  1. 125:1
  2. 130:1
  3. 135:1

Notes

Hemoglobin could not dissolve in water therefore PIs continued with BSA