User:Trisha I. Ibeh/Notebook/Trisha Notebook/2014/01/28: Difference between revisions
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Create BSA. AuNPs and magnetic BSA AuNPS | |||
# Synthesize BSA –AuNPs into test tube that will contain a total of 5mL | |||
# Au solution that has a final concentration of 0.25mM | |||
# BSA solution that has final concentrations that reflects the following Au:Protein ratios | |||
* 105 | |||
* 110 | |||
* 115 | |||
* 120 | |||
* 125 | |||
* 130 | |||
* 135 | |||
* 140 | |||
* 145 | |||
* 150 | |||
# Water up to 5mL | |||
# Put into incubator for 4 hours at 80 degrees Celsiuis | |||
# Add 30 mM FeSO4*7H2O, 400 mM KOH, and 100 mM KNO3 | |||
Separately make BSA- AuNPs the same way as in steps 1-4 but with ratios of: | |||
* 125:1 | |||
* 130:1 | |||
* 135:1 | |||
==Notes== | |||
Once the gold was added to the solution within the ratios of 105- 150, the solution immediately turned purple indicating that nanoparticles were formed. | |||
After about 10- 15 minutes, a green precipitate that was initially believed to be fibers were formed. This is due to the reduction of the gold by the FeSO4*7H2O | |||
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Revision as of 11:55, 30 January 2014
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BSA Magnetic Particles Day 5Create BSA. AuNPs and magnetic BSA AuNPS
Separately make BSA- AuNPs the same way as in steps 1-4 but with ratios of:
NotesOnce the gold was added to the solution within the ratios of 105- 150, the solution immediately turned purple indicating that nanoparticles were formed. After about 10- 15 minutes, a green precipitate that was initially believed to be fibers were formed. This is due to the reduction of the gold by the FeSO4*7H2O |