User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/09/04

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Objective

Finish up the lab from the previous day (9/3/13) by taking the UV- Vis spectrum of inosine and doing data analysis. From the data, a Grubb's test will be performed to determine the outliers. The concentration of the unknown sample will be determined ad the molar absorbtivity will also be determined from the class data.

Procedure

The same procedure that was performed with adenosine will be performed with inosine. The procedure can be found here.

Data

Inosine Abs Spectra

Screen Shot 2013-10-05 at 6.27.27 AM.png

Inosine Calibration Curve

Screen Shot 2013-10-05 at 6.13.30 AM.png

Class Data (pooled)

Abs mean and std dev (adenosine and inosine)

Notes

I made a stock solution of BSA today. Here is the info:

BSA solution 0.0104g BSA (MW = 66776g/mol) in 0.0100mL water → 15.6μM