User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/09/03
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The template for this lab can be seen from Dr. Hartings lab. Values are altered to accurately describe the lab that was conducted on this day. The template can be found here
The molar absorptivities of two different molecules, adenosine and inosine were determined in this lab using UV-Vis and Beer's law. The changes in UV-Vis spectra will be observed to determine changes in concentration of both adenosine and inosine. In order to do this, the molar absorptivity (ε) of both of these molecules will be known. A calibration curve from the class data will be created. From this data the standard deviation, Confidence Interval (90% and 95% confidence) will be calculated and Grubb's test will be performed to determine the outlier.
Stock solutions were made to create these dilutions for each molecule. The calculations for the stock solution and dilutions were performed before lab.
The RANDOM concentration was 0.25*10^-5 for adenosine and 0.4*10^-5 for inosine.
Preparation of Dilutions of Adenosine
Preparation of Dilutions of Inosine
Adenosine Absorbance Spectrum at 260 nm
Adenosine Calibration Curve at 260 nm
Class Calibration Curve for Adenosine at 259 nm
Due to the time restrictions of the lab, the inosine spectra was taken the next lab (9/4/13).
Grubb's test was conducted to determine the outliers in the pooled class data.