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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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Template for this lab is taken from Dr. Hartings. Values are altered to accurately describe the lab that was conducted on this day. The template can be found [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28 here] | |||
==Objective== | |||
Synthesize two different sets of gold nanoparticles. In one set, Au<sup>3+</sup> is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is surrounded and stabilized by BSA. In the second set, Au<sup>3+</sup> is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. | |||
==BSA-AuNP== | |||
Start by placing all of the materials into a volumetric flask. The initial protein and gold solutions were made by Dr. Hartings. | |||
This procedure was taken from the following [http://pubs.acs.org/doi/abs/10.1021/jp110296y reference]. | |||
# Add 1.2mL of the (~2.54mM -note the exact concentration) gold (HAuCl<sub>4</sub>) solution to a 10mL volumetric flask | |||
# Add an appropriate amount of BSA solution so that the final concentration of gold is 90X that of BSA | |||
# Add deionized water up to 10.2mL | |||
# Transfer solution to a test tube and cap with aluminum foil | |||
# Heat in oven at 80C for 3 hours | |||
# Transfer solution to a plastic falcon tube (with blue cap) | |||
<u>Stock solutions made</u> | |||
# Gold solution (HAuCl<sub>4</sub>·3H<sub>2</sub>O) 0.0100g in 0.0100mL water → 2.54mM | |||
# BSA solution 0.0104g BSA (MW = 66776g/mol) in 0.0100mL water → 15.6μM | |||
==citrate-AuNP== | |||
# Take 50mL of the .249mM of HAuCl<sub>4</sub> solution from the 250mL volumetric flask. | |||
# Heat this solution to boiling while stirring | |||
# Add <strike>3mL</strike> 1.5mL of 1% (w/v) sodium citrate | |||
# Boil solution for another 40 minutes | |||
# Cool to room temperature and measure the volume | |||
# Determine the final concentration of gold and citrate | |||
<u>Stock solutions made</u> | |||
# Gold solution (HAuCl<sub>4</sub>·3H<sub>2</sub>O) 0.0245g in 0.2505mL water → 0.249mM | |||
# Sodium Citrate (Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>·2H<sub>2</sub>O) 0.1010g in 10.0mL → 1.01% | |||
==Data== | |||
[[Category:Course]] | |||
[[Category:Miscellaneous]] | |||
<br>[[Image:Absorbtionvswavelength.png]] | |||
Peak Wavelength: 518 nm | |||
Absorbance at Peak: .612 | |||
In order to determine the concentration, the Absorbance at 450 is known. | |||
Abs (450): .389 | |||
Abs (518)/ Abs (450)= .612/ .389= 1.57 | |||
Using the data found [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf here] the value of 1.57 resulted in 12 d/nm. Again, using the link, this led to a molar absorptivity of 1*10^8. | |||
Using Beer's law and the newly found value for molar absorptivity, the concentration was determined to be 3.57*10^-9M. | |||
==Notes== | |||
The citrate- AuNPs were discarded as a result of it being spilled on the lab bench therefore it will be synthesized again in a future lab. | |||
As a result of the citrate being discarded, the citrate from Moira's group was used for the purposes of this lab. | |||
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Revision as of 00:05, 5 October 2013
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveSynthesize two different sets of gold nanoparticles. In one set, Au3+ is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is surrounded and stabilized by BSA. In the second set, Au3+ is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. BSA-AuNPStart by placing all of the materials into a volumetric flask. The initial protein and gold solutions were made by Dr. Hartings. This procedure was taken from the following reference.
Stock solutions made
citrate-AuNP
Data
Absorbance at Peak: .612 In order to determine the concentration, the Absorbance at 450 is known. Abs (450): .389 Abs (518)/ Abs (450)= .612/ .389= 1.57 Using the data found here the value of 1.57 resulted in 12 d/nm. Again, using the link, this led to a molar absorptivity of 1*10^8. Using Beer's law and the newly found value for molar absorptivity, the concentration was determined to be 3.57*10^-9M. NotesThe citrate- AuNPs were discarded as a result of it being spilled on the lab bench therefore it will be synthesized again in a future lab. As a result of the citrate being discarded, the citrate from Moira's group was used for the purposes of this lab.
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