User:Torsten Waldminghaus/flow cytometry notes

From OpenWetWare
< User:Torsten Waldminghaus
Revision as of 03:19, 2 April 2008 by Torsten Waldminghaus (talk | contribs) (New page: ==Purpose== * TBS stands for tris-buffered saline and is used in many protocols for washing ==Recipe== *1x TBS with 20mM Tris used for flow cytometry in Skarstad-Lab {|border="1" |+ ''...)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

Purpose

  • TBS stands for tris-buffered saline and is used in many protocols for washing


Recipe

  • 1x TBS with 20mM Tris used for flow cytometry in Skarstad-Lab
1xTBS
for 500 ml
10 ml 1M Tris-HCl pH 7.5
13 ml 5M NaCl
477 ml ddH2O
  • Prepare and sterile filter. Store at 4°C.