Difference between revisions of "User:Torsten Waldminghaus/flow cytometry notes"

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(New page: ==Purpose== * TBS stands for tris-buffered saline and is used in many protocols for washing ==Recipe== *1x TBS with 20mM Tris used for flow cytometry in Skarstad-Lab {|border="1" |+ ''...)
 
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==Purpose==
 
* TBS stands for tris-buffered saline and is used in many protocols for washing
 
 
 
 
==Recipe==
 
==Recipe==
  
 
*1x TBS with 20mM Tris used for flow cytometry in Skarstad-Lab
 
*1x TBS with 20mM Tris used for flow cytometry in Skarstad-Lab
 +
  
 
{|border="1"
 
{|border="1"

Revision as of 04:20, 2 April 2008

Recipe

  • 1x TBS with 20mM Tris used for flow cytometry in Skarstad-Lab


1xTBS
for 500 ml
10 ml 1M Tris-HCl pH 7.5
13 ml 5M NaCl
477 ml ddH2O
  • Prepare and sterile filter. Store at 4°C.