Difference between revisions of "User:Torsten Waldminghaus/Strains"

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(New page: {| border="3" ! Strain !! Parental strain !! Characteristics !! AB resistence !! Reference |- ! ''TW1'' | DH5α | ? |no | |- ! ''TW2'' |DH5α |pFH2102 |amp | |- ! ''TW3'' |MG1655 |? |no ...)
 
Line 2: Line 2:
 
! Strain !! Parental strain !! Characteristics !! AB resistence !! Reference
 
! Strain !! Parental strain !! Characteristics !! AB resistence !! Reference
 
|-
 
|-
! ''TW1''
+
! TW1
 
| DH5α  
 
| DH5α  
| ?
+
| supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 gyrA96
 
|no
 
|no
 
|
 
|
 
|-
 
|-
! ''TW2''
+
! TW2
 
|DH5α  
 
|DH5α  
 
|pFH2102
 
|pFH2102
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|
 
|
 
|-
 
|-
! ''TW3''
+
! TW3
 
|MG1655
 
|MG1655
 
|?
 
|?
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|
 
|
 
|-
 
|-
! ''TW4''
+
! TW4
 
|CM735
 
|CM735
 
|metE46 trp-3,his-4,thi-1,galK2,lacY1 or lacZ4,mth-1, ara-9,tsx-3,ton-1,rpsL8 or p,supE44 -
 
|metE46 trp-3,his-4,thi-1,galK2,lacY1 or lacZ4,mth-1, ara-9,tsx-3,ton-1,rpsL8 or p,supE44 -
 
|no
 
|no
|
+
|<cite>Hansen-1989</cite>
 
|-
 
|-
! ''TW5''
+
! TW5
 
|DH5α
 
|DH5α
 
|pGEM-pyrB
 
|pGEM-pyrB
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|
 
|
 
|-
 
|-
! ''TW6''
+
! TW6
 
|DH5α
 
|DH5α
 
|pFH2102-pyrB (=ppyrB)
 
|pFH2102-pyrB (=ppyrB)
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|
 
|
 
|-
 
|-
! ''TW7''
+
! TW7
 
|SG2221T  
 
|SG2221T  
 
|pFH2102
 
|pFH2102
 
|amp
 
|amp
 
|<cite>Hoskins-2000</cite>
 
|<cite>Hoskins-2000</cite>
 +
|-
 +
! TW8
 +
|MG1655
 +
|pyrB::Tn5
 +
|kan
 +
|Transfered to MG1655 by P1 transduction from ME8359 ([http://www.shigen.nig.ac.jp/ecoli/strain/top/top.jsp National BioResource Project E.coli Strain])
 +
|-
 +
! TW9
 +
|TW8
 +
|pyrB::Tn5 ppyrB
 +
|kan, amp
 +
|
 +
|-
 +
! TW10
 +
|TW8
 +
|pyrB::Tn5 pFH2102 (empty vector)
 +
|kan, amp
 +
|
 +
|-
 +
! TW11
 +
|TW3 (MG1655)
 +
|ppyrB
 +
|kan, amp
 +
|
 +
|-
 +
! TW12
 +
|TW3 (MG1655)
 +
|pFH2102 (empty vector)
 +
|kan, amp
 +
|
 
|-
 
|-
 
|}
 
|}
Line 49: Line 79:
 
<biblio>
 
<biblio>
 
#Hoskins-2000 pmid=10922051
 
#Hoskins-2000 pmid=10922051
 +
#Hansen-1984 pmid=6094968
 
</biblio>
 
</biblio>

Revision as of 05:34, 28 April 2008

Strain Parental strain Characteristics AB resistence Reference
TW1 DH5α supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 gyrA96 no
TW2 DH5α pFH2102 amp
TW3 MG1655 ? no
TW4 CM735 metE46 trp-3,his-4,thi-1,galK2,lacY1 or lacZ4,mth-1, ara-9,tsx-3,ton-1,rpsL8 or p,supE44 - no [1]
TW5 DH5α pGEM-pyrB amp
TW6 DH5α pFH2102-pyrB (=ppyrB) amp
TW7 SG2221T pFH2102 amp [2]
TW8 MG1655 pyrB::Tn5 kan Transfered to MG1655 by P1 transduction from ME8359 (National BioResource Project E.coli Strain)
TW9 TW8 pyrB::Tn5 ppyrB kan, amp
TW10 TW8 pyrB::Tn5 pFH2102 (empty vector) kan, amp
TW11 TW3 (MG1655) ppyrB kan, amp
TW12 TW3 (MG1655) pFH2102 (empty vector) kan, amp


  1. Hoskins JR, Singh SK, Maurizi MR, and Wickner S. Protein binding and unfolding by the chaperone ClpA and degradation by the protease ClpAP. Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8892-7. PubMed ID:10922051 | HubMed [Hoskins-2000]
  2. Hansen EB, Atlung T, Hansen FG, Skovgaard O, and von Meyenburg K. Fine structure genetic map and complementation analysis of mutations in the dnaA gene of Escherichia coli. Mol Gen Genet. 1984;196(3):387-96. PubMed ID:6094968 | HubMed [Hansen-1984]
All Medline abstracts: PubMed | HubMed