Difference between revisions of "User:Tom Adie/Sandbox/1"

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(New page: <html><TABLE WIDTH="80%" ><TR ><TD ><A NAME="TBL1" > </A ></TD ></TR ><TR ><TD ALIGN="CENTER" ><B >Table 1. </B ><B >Strains and plasmids used in this work.</B ></TD ></TR ><TR VALIGN...)
 
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<td ><A NAME="TBL1" >&nbsp;</A ></TD ></TR >
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<td ALIGN="CENTER" ><B >Table 1. </B ><B >Strains and plasmids used in this work.</B ></TD ></TR >
>&nbsp;</A
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<tr VALIGN="BOTTOM" >
></TD
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<td >
></TR
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<table >
><B
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<tr >
>Table 1. </B
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<th ALIGN="left" VALIGN="TOP" >
><B
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>Strains and plasmids used in this work.</B
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<p>Strain or plasmid</TH >
></TD
+
<th ALIGN="left" VALIGN="TOP" >
></TR
+
 
><TR
+
<p>Relevant properties or genotype</TH >
VALIGN="BOTTOM"
+
<th ALIGN="left" VALIGN="TOP" ><NOBR >
><TD
+
 
><HR
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<p>Source</NOBR ></TH ></TR >
size="2"
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<tr >
noshade="1"></TD
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<td COLSPAN="3" >
></TR
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<hr size="1"></TD ></TR >
><TR
+
<tr >
><TD
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<td ALIGN="left" VALIGN="TOP" ><NOBR >
ALIGN="CENTER"
+
 
><TABLE
+
<p>Strains</NOBR ></TD >
><TR
+
<td ALIGN="left" VALIGN="TOP" ><NOBR ></NOBR ></TD >
><TH
+
<td ALIGN="left" VALIGN="TOP" ><NOBR ></NOBR ></TD ></TR >
ALIGN="left"
+
<tr >
VALIGN="TOP"
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<td ALIGN="left" VALIGN="TOP" >
><P>Strain or plasmid</TH
+
 
><TH
+
<p><I >E. coli</I > DH5<IMG SRC="/giflibrary/12/alpha.gif" BORDER="0"></TD >
ALIGN="left"
+
<td ALIGN="left" VALIGN="TOP" >
VALIGN="TOP"
+
 
><P>Relevant properties or genotype</TH
+
<p>F<SUP >-</SUP >, <I ><IMG SRC="/giflibrary/12/phgr.gif" BORDER="0" ALIGN="ABSBOTTOM"></I >80d, <I >lacZ</I ><IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, <I >endA</I >1, <I >recA</I >1, <I >hsdR</I >17(r<SUB >K</SUB ><SUP >-</SUP >m<SUB >K</SUB ><SUP >-</SUP >), <I >supE</I >44, <I >thi</I >-1, <I >gyrA</I >96, <I >relA</I >1, <IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">(<I >lacZYA-argF</I >)U169</TD >
><TH
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
ALIGN="left"
+
 
VALIGN="TOP"
+
<p>[a]</NOBR ></TD ></TR >
><NOBR
+
<tr >
><P>Source</NOBR
+
<td ALIGN="left" VALIGN="TOP" >
></TH
+
 
></TR
+
<p><I >E. coli</I > M15 [pREP4]</TD >
><TR
+
<td ALIGN="left" VALIGN="TOP" >
><TD
+
 
COLSPAN="3"
+
<p>F<SUP >-</SUP >, <I >lacZ<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, thi</I >-1, <I >lac<SUP >-</SUP ></I >, <I >mtl<SUP >-</SUP ></I >, <I >recA<SUP >+</SUP ></I > , Km<SUP >R</SUP ></TD >
><HR
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
size="1"></TD
+
 
></TR
+
<p>[a]</NOBR ></TD ></TR >
><TR
+
<tr >
><TD
+
<td ALIGN="left" VALIGN="TOP" >
ALIGN="left"
+
 
VALIGN="TOP"
+
<p><I >Pseudomonas putida</I > KT2440</TD >
><NOBR
+
<td ALIGN="left" VALIGN="TOP" >
><P>Strains</NOBR
+
 
></TD
+
<p>type strain</TD >
><TD
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
ALIGN="left"
+
 
VALIGN="TOP"
+
<p>[b]</NOBR ></TD ></TR >
><NOBR
+
<tr >
></NOBR
+
<td ALIGN="left" VALIGN="TOP" >
></TD
+
 
><TD
+
<p><I >Synechocystis</I > sp. PCC 6803</TD >
ALIGN="left"
+
<td ALIGN="left" VALIGN="TOP" >
VALIGN="TOP"
+
 
><NOBR
+
<p>type strain</TD >
></NOBR
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
></TD
+
 
></TR
+
<p>[c]</NOBR ></TD ></TR >
><TR
+
<tr >
><TD
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
ALIGN="left"
+
 
VALIGN="TOP"
+
<p>Plasmids</NOBR ></TD >
><P><I
+
<td ALIGN="left" VALIGN="TOP" ><NOBR ></NOBR ></TD >
>E. coli</I
+
<td ALIGN="left" VALIGN="TOP" ><NOBR ></NOBR ></TD ></TR >
> DH5<IMG
+
<tr >
SRC="/giflibrary/12/alpha.gif"
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
BORDER="0"></TD
+
 
><TD
+
<p>pCAR16</NOBR ></TD >
ALIGN="left"
+
<td ALIGN="left" VALIGN="TOP" >
VALIGN="TOP"
+
 
><P>F<SUP
+
<p><IMG SRC="/giflibrary/12/beta.gif" BORDER="0" ALIGN="ABSBOTTOM">-carotene biosynthesis gene cluster from <I >Pantoea ananatis</I > in pUC19, Amp<SUP >R</SUP ></TD >
>-</SUP
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
>, <I
+
 
><IMG
+
<p>[<A HREF="#BIB27" >27</A >]</NOBR ></TD ></TR >
SRC="/giflibrary/12/phgr.gif"
+
<tr >
BORDER="0"
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
ALIGN="ABSBOTTOM"></I
+
 
>80d, <I
+
<p>pJF119EH</NOBR ></TD >
>lacZ</I
+
<td ALIGN="left" VALIGN="TOP" >
><IMG
+
 
SRC="/giflibrary/12/Delta.gif"
+
<p>cloning vector, tac-promoter, IPTG inducible, Amp<SUP >R</SUP ></TD >
BORDER="0">M15, <I
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
>endA</I
+
 
>1, <I
+
<p>[<A HREF="#BIB25" >25</A >]</NOBR ></TD ></TR >
>recA</I
+
<tr >
>1, <I
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
>hsdR</I
+
 
>17(r<SUB
+
<p>pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</NOBR ></TD >
>K</SUB
+
<td ALIGN="left" VALIGN="TOP" >
><SUP
+
 
>-</SUP
+
<p>cloning vector, RBS, tac-promoter, IPTG inducible, Amp<SUP >R</SUP ></TD >
>m<SUB
+
<td ALIGN="left" VALIGN="TOP" >
>K</SUB
+
 
><SUP
+
<p>this study</TD ></TR >
>-</SUP
+
<tr >
>), <I
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
>supE</I
+
 
>44, <I
+
<p>pAW229</NOBR ></TD >
>thi</I
+
<td ALIGN="left" VALIGN="TOP" >
>-1, <I
+
 
>gyrA</I
+
<p>cloning vector, RBS, <I >rha</I >-promoter, <FONT SIZE="-1" >L</FONT >-rhamnose inducible, Cm<SUP >R</SUP ></TD >
>96, <I
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
>relA</I
+
 
>1, <IMG
+
<p>[<A HREF="#BIB26" >26</A >]</NOBR ></TD ></TR >
SRC="/giflibrary/12/Delta.gif"
+
<tr >
BORDER="0">(<I
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
>lacZYA-argF</I
+
 
>)U169</TD
+
<p>pQE31-VTE1</NOBR ></TD >
><TD
+
<td ALIGN="left" VALIGN="TOP" >
ALIGN="left"
+
 
VALIGN="TOP"
+
<p>tocopherol-cyclase (vte1) from <I >Arabidopsis thaliana</I > in pQE31, IPTG inducible, Amp<SUP >R</SUP ></TD >
><NOBR
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
><P>[a]</NOBR
+
 
></TD
+
<p>[<A HREF="#BIB18A" >18a</A >]</NOBR ></TD ></TR >
></TR
+
<tr >
><TR
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
><TD
+
 
ALIGN="left"
+
<p>pCAS2JF</NOBR ></TD >
VALIGN="TOP"
+
<td ALIGN="left" VALIGN="TOP" >
><P><I
+
 
>E. coli</I
+
<p>1.2 kb EcoRI/BamHI PCR fragment <I >hpd</I > in pJF119EH</TD >
> M15 [pREP4]</TD
+
<td ALIGN="left" VALIGN="TOP" >
><TD
+
 
ALIGN="left"
+
<p>this study</TD ></TR >
VALIGN="TOP"
+
<tr >
><P>F<SUP
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
>-</SUP
+
 
>, <I
+
<p>pCAS8</NOBR ></TD >
>lacZ<IMG
+
<td ALIGN="left" VALIGN="TOP" >
SRC="/giflibrary/12/Delta.gif"
+
 
BORDER="0">M15, thi</I
+
<p>1.1 kb NdeI/BamHI PCR fragment <I >ggh</I > in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD >
>-1, <I
+
<td ALIGN="left" VALIGN="TOP" >
>lac<SUP
+
 
>-</SUP
+
<p>this study</TD ></TR >
></I
+
<tr >
>, <I
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
>mtl<SUP
+
 
>-</SUP
+
<p>pCAS11</NOBR ></TD >
></I
+
<td ALIGN="left" VALIGN="TOP" >
>, <I
+
 
>recA<SUP
+
<p>0.9 kb BglII/BamHI PCR fragment <I >crtE</I > in pCAS8</TD >
>+</SUP
+
<td ALIGN="left" VALIGN="TOP" >
></I
+
 
> , Km<SUP
+
<p>this study</TD ></TR >
>R</SUP
+
<tr >
></TD
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
><TD
+
 
ALIGN="left"
+
<p>pCAS12</NOBR ></TD >
VALIGN="TOP"
+
<td ALIGN="left" VALIGN="TOP" >
><NOBR
+
 
><P>[a]</NOBR
+
<p>1.0 kb BglII/BamHI PCR fragment <I >hpt</I > in pCAS11</TD >
></TD
+
<td ALIGN="left" VALIGN="TOP" >
></TR
+
 
><TR
+
<p>this study</TD ></TR >
><TD
+
<tr >
ALIGN="left"
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
VALIGN="TOP"
+
 
><P><I
+
<p>pCAS15</NOBR ></TD >
>Pseudomonas putida</I
+
<td ALIGN="left" VALIGN="TOP" >
> KT2440</TD
+
 
><TD
+
<p>1.2 kb NdeI/BamHI PCR fragment <I >hpd</I > in pAW229</TD >
ALIGN="left"
+
<td ALIGN="left" VALIGN="TOP" >
VALIGN="TOP"
+
 
><P>type strain</TD
+
<p>this study</TD ></TR >
><TD
+
<tr >
ALIGN="left"
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
VALIGN="TOP"
+
 
><NOBR
+
<p>pCAS18</NOBR ></TD >
><P>[b]</NOBR
+
<td ALIGN="left" VALIGN="TOP" >
></TD
+
 
></TR
+
<p>1.2 kb BglII/BamHI PCR fragment <I >hpd</I > in pCAS11</TD >
><TR
+
<td ALIGN="left" VALIGN="TOP" >
><TD
+
 
ALIGN="left"
+
<p>this study</TD ></TR >
VALIGN="TOP"
+
<tr >
><P><I
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
>Synechocystis</I
+
 
> sp. PCC 6803</TD
+
<p>pCAS19</NOBR ></TD >
><TD
+
<td ALIGN="left" VALIGN="TOP" >
ALIGN="left"
+
 
VALIGN="TOP"
+
<p>1.0 kb NdeI/BamHI PCR fragment <I >hpt</I > in pAW229</TD >
><P>type strain</TD
+
<td ALIGN="left" VALIGN="TOP" >
><TD
+
 
ALIGN="left"
+
<p>this study</TD ></TR >
VALIGN="TOP"
+
<tr >
><NOBR
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
><P>[c]</NOBR
+
 
></TD
+
<p>pCAS23</NOBR ></TD >
></TR
+
<td ALIGN="left" VALIGN="TOP" >
><TR
+
 
><TD
+
<p>1.0 kb NdeI/BamHI PCR fragment <I >hpt</I > in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD >
ALIGN="left"
+
<td ALIGN="left" VALIGN="TOP" >
VALIGN="TOP"
+
 
><NOBR
+
<p>this study</TD ></TR >
><P>Plasmids</NOBR
+
<tr >
></TD
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
><TD
+
 
ALIGN="left"
+
<p>pCAS24</NOBR ></TD >
VALIGN="TOP"
+
<td ALIGN="left" VALIGN="TOP" >
><NOBR
+
 
></NOBR
+
<p>0.9 kb NdeI/BamHI PCR fragment <I >crtE</I > in pCAS23</TD >
></TD
+
<td ALIGN="left" VALIGN="TOP" >
><TD
+
 
ALIGN="left"
+
<p>this study</TD ></TR >
VALIGN="TOP"
+
<tr >
><NOBR
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
></NOBR
+
 
></TD
+
<p>pCAS27</NOBR ></TD >
></TR
+
<td ALIGN="left" VALIGN="TOP" >
><TR
+
 
><TD
+
<p>1.1 kb NdeI/BamHI PCR fragment <I >cyc-sy</I > in pAW229</TD >
ALIGN="left"
+
<td ALIGN="left" VALIGN="TOP" >
VALIGN="TOP"
+
 
><NOBR
+
<p>this study</TD ></TR >
><P>pCAR16</NOBR
+
<tr >
></TD
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
><TD
+
 
ALIGN="left"
+
<p>pCAS29</NOBR ></TD >
VALIGN="TOP"
+
<td ALIGN="left" VALIGN="TOP" >
><P><IMG
+
 
SRC="/giflibrary/12/beta.gif"
+
<p>1.2 kb BglII/BamHI PCR fragment <I >hpd</I > in pCAS18</TD >
BORDER="0"
+
<td ALIGN="left" VALIGN="TOP" >
ALIGN="ABSBOTTOM">-carotene biosynthesis gene cluster from <I
+
 
>Pantoea ananatis</I
+
<p>this study</TD ></TR >
> in pUC19, Amp<SUP
+
<tr >
>R</SUP
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
></TD
+
 
><TD
+
<p>pCAS30</NOBR ></TD >
ALIGN="left"
+
<td ALIGN="left" VALIGN="TOP" >
VALIGN="TOP"
+
 
><NOBR
+
<p>0.9 kb NdeI/BamHI PCR fragment <I >crtE</I > in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD >
><P>[<A
+
<td ALIGN="left" VALIGN="TOP" >
HREF="#BIB27"
+
 
>27</A
+
<p>this study</TD ></TR >
>]</NOBR
+
<tr >
></TD
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
></TR
+
 
><TR
+
<p>pCAS37</NOBR ></TD >
><TD
+
<td ALIGN="left" VALIGN="TOP" >
ALIGN="left"
+
 
VALIGN="TOP"
+
<p>1.2 kb BglII/BamHI PCR fragment <I >hpd</I > in pCAS24</TD >
><NOBR
+
<td ALIGN="left" VALIGN="TOP" >
><P>pJF119EH</NOBR
+
 
></TD
+
<p>this study</TD ></TR >
><TD
+
<tr >
ALIGN="left"
+
<td ALIGN="left" VALIGN="TOP" ><NOBR >
VALIGN="TOP"
+
 
><P>cloning vector, tac-promoter, IPTG inducible, Amp<SUP
+
<p>pCAS47</NOBR ></TD >
>R</SUP
+
<td ALIGN="left" VALIGN="TOP" >
></TD
+
 
><TD
+
<p>1.3 kb BglII PCR fragment <I >vte1</I > in pCAS29</TD >
ALIGN="left"
+
<td ALIGN="left" VALIGN="TOP" >
VALIGN="TOP"
+
 
><NOBR
+
<p>this study</TD ></TR ></TABLE ></TD ></TR >
><P>[<A
+
<tr VALIGN="BOTTOM" >
HREF="#BIB25"
+
<td >
>25</A
+
<hr size="2" noshade="1"></TD ></TR ></TABLE ></CENTER ><CENTER >
>]</NOBR
+
<table WIDTH="80%" >
></TD
+
<tr >
></TR
+
<td ><FONT SIZE="-1" >&nbsp;&nbsp;&nbsp;[a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universit&auml;t-Gie&szlig;en (Germany).
><TR
+
<br></FONT ></TD ></TR ></TABLE >
><TD
+
</html>
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>pJF119<IMG
 
SRC="/giflibrary/12/Delta.gif"
 
BORDER="0">N</NOBR
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>cloning vector, RBS, tac-promoter, IPTG inducible, Amp<SUP
 
>R</SUP
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>this study</TD
 
></TR
 
><TR
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>pAW229</NOBR
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>cloning vector, RBS, <I
 
>rha</I
 
>-promoter, <FONT
 
SIZE="-1"
 
>L</FONT
 
>-rhamnose inducible, Cm<SUP
 
>R</SUP
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>[<A
 
HREF="#BIB26"
 
>26</A
 
>]</NOBR
 
></TD
 
></TR
 
><TR
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>pQE31-VTE1</NOBR
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>tocopherol-cyclase (vte1) from <I
 
>Arabidopsis thaliana</I
 
> in pQE31, IPTG inducible, Amp<SUP
 
>R</SUP
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>[<A
 
HREF="#BIB18A"
 
>18a</A
 
>]</NOBR
 
></TD
 
></TR
 
><TR
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>pCAS2JF</NOBR
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>1.2 kb EcoRI/BamHI PCR fragment <I
 
>hpd</I
 
> in pJF119EH</TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>this study</TD
 
></TR
 
><TR
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>pCAS8</NOBR
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>1.1 kb NdeI/BamHI PCR fragment <I
 
>ggh</I
 
> in pJF119<IMG
 
SRC="/giflibrary/12/Delta.gif"
 
BORDER="0">N</TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>this study</TD
 
></TR
 
><TR
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>pCAS11</NOBR
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>0.9 kb BglII/BamHI PCR fragment <I
 
>crtE</I
 
> in pCAS8</TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>this study</TD
 
></TR
 
><TR
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>pCAS12</NOBR
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>1.0 kb BglII/BamHI PCR fragment <I
 
>hpt</I
 
> in pCAS11</TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>this study</TD
 
></TR
 
><TR
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>pCAS15</NOBR
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>1.2 kb NdeI/BamHI PCR fragment <I
 
>hpd</I
 
> in pAW229</TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>this study</TD
 
></TR
 
><TR
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>pCAS18</NOBR
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>1.2 kb BglII/BamHI PCR fragment <I
 
>hpd</I
 
> in pCAS11</TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>this study</TD
 
></TR
 
><TR
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>pCAS19</NOBR
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>1.0 kb NdeI/BamHI PCR fragment <I
 
>hpt</I
 
> in pAW229</TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>this study</TD
 
></TR
 
><TR
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>pCAS23</NOBR
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>1.0 kb NdeI/BamHI PCR fragment <I
 
>hpt</I
 
> in pJF119<IMG
 
SRC="/giflibrary/12/Delta.gif"
 
BORDER="0">N</TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>this study</TD
 
></TR
 
><TR
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>pCAS24</NOBR
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>0.9 kb NdeI/BamHI PCR fragment <I
 
>crtE</I
 
> in pCAS23</TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>this study</TD
 
></TR
 
><TR
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>pCAS27</NOBR
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>1.1 kb NdeI/BamHI PCR fragment <I
 
>cyc-sy</I
 
> in pAW229</TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>this study</TD
 
></TR
 
><TR
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>pCAS29</NOBR
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>1.2 kb BglII/BamHI PCR fragment <I
 
>hpd</I
 
> in pCAS18</TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>this study</TD
 
></TR
 
><TR
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>pCAS30</NOBR
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>0.9 kb NdeI/BamHI PCR fragment <I
 
>crtE</I
 
> in pJF119<IMG
 
SRC="/giflibrary/12/Delta.gif"
 
BORDER="0">N</TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>this study</TD
 
></TR
 
><TR
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>pCAS37</NOBR
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>1.2 kb BglII/BamHI PCR fragment <I
 
>hpd</I
 
> in pCAS24</TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>this study</TD
 
></TR
 
><TR
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><NOBR
 
><P>pCAS47</NOBR
 
></TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>1.3 kb BglII PCR fragment <I
 
>vte1</I
 
> in pCAS29</TD
 
><TD
 
ALIGN="left"
 
VALIGN="TOP"
 
><P>this study</TD
 
></TR
 
></TABLE
 
></TD
 
></TR
 
><TR
 
VALIGN="BOTTOM"
 
><TD
 
><HR
 
size="2"
 
noshade="1"></TD
 
></TR
 
></TABLE
 
></CENTER
 
><CENTER
 
><TABLE
 
WIDTH="80%"
 
><TR
 
><TD
 
><FONT
 
SIZE="-1"
 
>&nbsp;&nbsp;&nbsp;[a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universit&auml;t-Gie&szlig;en (Germany).<BR></FONT
 
></TD
 
></TR
 
></TABLE
 
></html>
 

Revision as of 18:01, 19 July 2009

<html> <table WIDTH="80%" > <tr > <td ><A NAME="TBL1" >&nbsp;</A ></TD ></TR > <tr > <td ALIGN="CENTER" ><B >Table 1. </B ><B >Strains and plasmids used in this work.</B ></TD ></TR > <tr VALIGN="BOTTOM" > <td > <hr size="2" noshade="1"></TD ></TR > <tr > <td ALIGN="CENTER" > <table > <tr > <th ALIGN="left" VALIGN="TOP" >

<p>Strain or plasmid</TH > <th ALIGN="left" VALIGN="TOP" >

<p>Relevant properties or genotype</TH > <th ALIGN="left" VALIGN="TOP" ><NOBR >

<p>Source</NOBR ></TH ></TR > <tr > <td COLSPAN="3" > <hr size="1"></TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>Strains</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" ><NOBR ></NOBR ></TD > <td ALIGN="left" VALIGN="TOP" ><NOBR ></NOBR ></TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" >

<p><I >E. coli</I > DH5<IMG SRC="/giflibrary/12/alpha.gif" BORDER="0"></TD > <td ALIGN="left" VALIGN="TOP" >

<p>F<SUP >-</SUP >, <I ><IMG SRC="/giflibrary/12/phgr.gif" BORDER="0" ALIGN="ABSBOTTOM"></I >80d, <I >lacZ</I ><IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, <I >endA</I >1, <I >recA</I >1, <I >hsdR</I >17(r<SUB >K</SUB ><SUP >-</SUP >m<SUB >K</SUB ><SUP >-</SUP >), <I >supE</I >44, <I >thi</I >-1, <I >gyrA</I >96, <I >relA</I >1, <IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">(<I >lacZYA-argF</I >)U169</TD > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>[a]</NOBR ></TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" >

<p><I >E. coli</I > M15 [pREP4]</TD > <td ALIGN="left" VALIGN="TOP" >

<p>F<SUP >-</SUP >, <I >lacZ<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">M15, thi</I >-1, <I >lac<SUP >-</SUP ></I >, <I >mtl<SUP >-</SUP ></I >, <I >recA<SUP >+</SUP ></I > , Km<SUP >R</SUP ></TD > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>[a]</NOBR ></TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" >

<p><I >Pseudomonas putida</I > KT2440</TD > <td ALIGN="left" VALIGN="TOP" >

<p>type strain</TD > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>[b]</NOBR ></TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" >

<p><I >Synechocystis</I > sp. PCC 6803</TD > <td ALIGN="left" VALIGN="TOP" >

<p>type strain</TD > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>[c]</NOBR ></TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>Plasmids</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" ><NOBR ></NOBR ></TD > <td ALIGN="left" VALIGN="TOP" ><NOBR ></NOBR ></TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pCAR16</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p><IMG SRC="/giflibrary/12/beta.gif" BORDER="0" ALIGN="ABSBOTTOM">-carotene biosynthesis gene cluster from <I >Pantoea ananatis</I > in pUC19, Amp<SUP >R</SUP ></TD > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>[<A HREF="#BIB27" >27</A >]</NOBR ></TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pJF119EH</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>cloning vector, tac-promoter, IPTG inducible, Amp<SUP >R</SUP ></TD > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>[<A HREF="#BIB25" >25</A >]</NOBR ></TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>cloning vector, RBS, tac-promoter, IPTG inducible, Amp<SUP >R</SUP ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>this study</TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pAW229</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>cloning vector, RBS, <I >rha</I >-promoter, <FONT SIZE="-1" >L</FONT >-rhamnose inducible, Cm<SUP >R</SUP ></TD > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>[<A HREF="#BIB26" >26</A >]</NOBR ></TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pQE31-VTE1</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>tocopherol-cyclase (vte1) from <I >Arabidopsis thaliana</I > in pQE31, IPTG inducible, Amp<SUP >R</SUP ></TD > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>[<A HREF="#BIB18A" >18a</A >]</NOBR ></TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pCAS2JF</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>1.2 kb EcoRI/BamHI PCR fragment <I >hpd</I > in pJF119EH</TD > <td ALIGN="left" VALIGN="TOP" >

<p>this study</TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pCAS8</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>1.1 kb NdeI/BamHI PCR fragment <I >ggh</I > in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD > <td ALIGN="left" VALIGN="TOP" >

<p>this study</TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pCAS11</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>0.9 kb BglII/BamHI PCR fragment <I >crtE</I > in pCAS8</TD > <td ALIGN="left" VALIGN="TOP" >

<p>this study</TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pCAS12</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>1.0 kb BglII/BamHI PCR fragment <I >hpt</I > in pCAS11</TD > <td ALIGN="left" VALIGN="TOP" >

<p>this study</TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pCAS15</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>1.2 kb NdeI/BamHI PCR fragment <I >hpd</I > in pAW229</TD > <td ALIGN="left" VALIGN="TOP" >

<p>this study</TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pCAS18</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>1.2 kb BglII/BamHI PCR fragment <I >hpd</I > in pCAS11</TD > <td ALIGN="left" VALIGN="TOP" >

<p>this study</TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pCAS19</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>1.0 kb NdeI/BamHI PCR fragment <I >hpt</I > in pAW229</TD > <td ALIGN="left" VALIGN="TOP" >

<p>this study</TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pCAS23</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>1.0 kb NdeI/BamHI PCR fragment <I >hpt</I > in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD > <td ALIGN="left" VALIGN="TOP" >

<p>this study</TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pCAS24</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>0.9 kb NdeI/BamHI PCR fragment <I >crtE</I > in pCAS23</TD > <td ALIGN="left" VALIGN="TOP" >

<p>this study</TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pCAS27</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>1.1 kb NdeI/BamHI PCR fragment <I >cyc-sy</I > in pAW229</TD > <td ALIGN="left" VALIGN="TOP" >

<p>this study</TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pCAS29</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>1.2 kb BglII/BamHI PCR fragment <I >hpd</I > in pCAS18</TD > <td ALIGN="left" VALIGN="TOP" >

<p>this study</TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pCAS30</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>0.9 kb NdeI/BamHI PCR fragment <I >crtE</I > in pJF119<IMG SRC="/giflibrary/12/Delta.gif" BORDER="0">N</TD > <td ALIGN="left" VALIGN="TOP" >

<p>this study</TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pCAS37</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>1.2 kb BglII/BamHI PCR fragment <I >hpd</I > in pCAS24</TD > <td ALIGN="left" VALIGN="TOP" >

<p>this study</TD ></TR > <tr > <td ALIGN="left" VALIGN="TOP" ><NOBR >

<p>pCAS47</NOBR ></TD > <td ALIGN="left" VALIGN="TOP" >

<p>1.3 kb BglII PCR fragment <I >vte1</I > in pCAS29</TD > <td ALIGN="left" VALIGN="TOP" >

<p>this study</TD ></TR ></TABLE ></TD ></TR > <tr VALIGN="BOTTOM" > <td > <hr size="2" noshade="1"></TD ></TR ></TABLE ></CENTER ><CENTER > <table WIDTH="80%" > <tr > <td ><FONT SIZE="-1" >&nbsp;&nbsp;&nbsp;[a] Commercial strain. [b] Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). [c] A kind gift from Prof. Forchhammer, Universit&auml;t-Gie&szlig;en (Germany). <br></FONT ></TD ></TR ></TABLE > </html>