Difference between revisions of "User:Tk/Notebook/MF/2010/06/12"

From OpenWetWare
< User:Tk‎ | Notebook‎ | MF‎ | 2010‎ | 06
Jump to: navigation, search
(Autocreate 2010/06/12 Entry for User:Tk/Notebook/MF)
 
(Title)
 
Line 6: Line 6:
 
| colspan="2"|
 
| colspan="2"|
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Title==
+
==Desired parts==
* Content
+
* Mflorum optimized genes
 +
** CFP
 +
** YFP
 +
** RFP
 +
** LacZ
 +
** cat (probably from Sebastian)
 +
** I-sceI enzyme
 +
* Cre-lox sites (maybe from Sebastian)
 +
* I-sceI cut site
 +
* E. coli optimized mfl304
 +
* modified pheS (already exists?)
 +
* up and down sequences around RE gene sites
  
 +
* modified plasmid containing left and right transposon sites (M1000 and M1001) with pvuII sites
 +
 +
Eliminate sites for PvuII, NheI, bibrick sites,
  
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Latest revision as of 04:26, 12 June 2010

Owwnotebook icon.png Mesoplasma florum simplification <html><img src="/images/9/94/Report.png" border="0" /></html> Main Page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Desired parts

  • Mflorum optimized genes
    • CFP
    • YFP
    • RFP
    • LacZ
    • cat (probably from Sebastian)
    • I-sceI enzyme
  • Cre-lox sites (maybe from Sebastian)
  • I-sceI cut site
  • E. coli optimized mfl304
  • modified pheS (already exists?)
  • up and down sequences around RE gene sites
  • modified plasmid containing left and right transposon sites (M1000 and M1001) with pvuII sites

Eliminate sites for PvuII, NheI, bibrick sites,