Difference between revisions of "User:Timothy L. Foley/Notebook/refolding matrix/2009/10/13/Refolding Matrix-10-13-2009"

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{| {{table}}
{|{{table}} width="800"
+
| align="center" style="background:#f0f0f0;"|'''Buffer #'''
 +
| align="center" style="background:#f0f0f0;"|'''pH'''
 +
| align="center" style="background:#f0f0f0;"|'''detergent'''
 +
| align="center" style="background:#f0f0f0;"|'''amino acids'''
 +
| align="center" style="background:#f0f0f0;"|'''salt'''
 +
| align="center" style="background:#f0f0f0;"|'''crowders'''
 +
| align="center" style="background:#f0f0f0;"|'''divalent cation'''
 +
| align="center" style="background:#f0f0f0;"|'''GnCl'''
 +
| align="center" style="background:#f0f0f0;"|'''BSA'''
 +
| align="center" style="background:#f0f0f0;"|'''Ligand'''
 +
| align="center" style="background:#f0f0f0;"|'''reducing agent'''
 +
| align="center" style="background:#f0f0f0;"|'''Y'''
 +
|-
 +
| 1||5.5 MES||none||none||low||PEG||1||0||1||0||DTT||
 +
|-
 +
| 2||5.5 MES||none||none||high||none||0||0||0||0||DTT||
 +
|-
 +
| 3||5.5 MES||none||none||high||PEG||0||0||0||1||none||
 +
|-
 +
| 4||5.5 MES||none||Arg||none||PEG||1||0||0||1||TCEP||
 +
|-
 +
| 5||5.5 MES||none||Glut||low||Glycerol||1||0||1||0||TCEP||
 +
|-
 +
| 6||5.5 MES||TritonX||Arg||none||Glycerol||1||0||1||1||none||
 +
|-
 +
| 7||5.5 MES||TritonX||Arg||high||none||0||0||1||0||TCEP||
 +
|-
 +
| 8||5.5 MES||TritonX||Glut||none||PEG||0||0||1||0||DTT||
 +
|-
 +
| 9||5.5 MES||TritonX||Glut||low||none||0||1||0||1||DTT||
 +
|-
 +
| 10||5.5 MES||TritonX||Glut||low||Glycerol||0||1||0||1||none||
 +
|-
 +
| 11||5.5 MES||Tween80||none||low||Glycerol||1||1||1||0||TCEP||
 +
|-
 +
| 12||5.5 MES||Tween80||Arg||low||none||0||0||0||0||DTT||
 +
|-
 +
| 13||5.5 MES||Tween80||Arg||high||Glycerol||0||1||1||1||TCEP||
 +
|-
 +
| 14||5.5 MES||Tween80||Glut||none||Glycerol||1||1||0||1||TCEP||
 +
|-
 +
| 15||5.5 MES||Tween80||Glut||high||PEG||0||1||1||1||none||
 +
|-
 +
| 16||5.5 MES||CHAPS||none||low||none||0||1||0||0||none||
 +
|-
 +
| 17||5.5 MES||CHAPS||none||high||none||0||1||1||1||TCEP||
 +
|-
 +
| 18||5.5 MES||CHAPS||none||high||none||1||1||0||1||DTT||
 +
|-
 +
| 19||5.5 MES||CHAPS||Arg||none||PEG||1||0||0||0||none||
 +
|-
 +
| 20||5.5 MES||CHAPS||Glut||none||Glycerol||1||1||1||0||DTT||
 +
|-
 +
| 21||6.5 MES||none||none||none||none||1||0||0||1||none||
 +
|-
 +
| 22||6.5 MES||none||none||none||Glycerol||0||1||1||0||DTT||
 +
|-
 +
| 23||6.5 MES||none||Arg||low||none||1||1||1||1||none||
 +
|-
 +
| 24||6.5 MES||none||Arg||high||PEG||1||1||1||0||none||
 +
|-
 +
| 25||6.5 MES||none||Glut||low||Glycerol||1||0||1||1||DTT||
 +
|-
 +
| 26||6.5 MES||TritonX||none||none||Glycerol||1||0||0||0||TCEP||
 +
|-
 +
| 27||6.5 MES||TritonX||Arg||low||PEG||0||1||1||1||DTT||
 +
|-
 +
| 28||6.5 MES||TritonX||Arg||high||Glycerol||0||0||0||0||DTT||
 +
|-
 +
| 29||6.5 MES||TritonX||Glut||low||none||1||1||0||0||TCEP||
 +
|-
 +
| 30||6.5 MES||TritonX||Glut||high||Glycerol||1||1||0||1||none||
 +
|-
 +
| 31||6.5 MES||Tween80||none||none||none||0||1||1||1||DTT||
 +
|-
 +
| 32||6.5 MES||Tween80||none||low||PEG||1||0||0||0||TCEP||
 +
|-
 +
| 33||6.5 MES||Tween80||none||low||PEG||1||0||0||1||DTT||
 +
|-
 +
| 34||6.5 MES||Tween80||Arg||none||PEG||0||1||1||0||TCEP||
 +
|-
 +
| 35||6.5 MES||Tween80||Glut||high||none||0||0||1||0||DTT||
 +
|-
 +
| 36||6.5 MES||CHAPS||none||high||Glycerol||0||1||1||0||TCEP||
 +
|-
 +
| 37||6.5 MES||CHAPS||Arg||high||PEG||1||1||1||0||none||
 +
|-
 +
| 38||6.5 MES||CHAPS||Arg||high||Glycerol||0||0||1||1||none||
 +
|-
 +
| 39||6.5 MES||CHAPS||Glut||none||none||1||1||0||1||none||
 +
|-
 +
| 40||6.5 MES||CHAPS||Glut||low||PEG||0||0||0||1||TCEP||
 +
|-
 +
| 41||7.5 HEPES||none||none||low||PEG||0||1||0||1||none||
 +
|-
 +
| 42||7.5 HEPES||none||Arg||high||none||0||0||1||1||TCEP||
 +
|-
 +
| 43||7.5 HEPES||none||Arg||high||Glycerol||0||1||0||0||none||
 +
|-
 +
| 44||7.5 HEPES||none||Glut||none||PEG||0||1||0||0||TCEP||
 +
|-
 +
| 45||7.5 HEPES||none||Glut||high||Glycerol||0||0||0||0||DTT||
 +
|-
 +
| 46||7.5 HEPES||TritonX||none||none||none||0||0||1||1||none||
 +
|-
 +
| 47||7.5 HEPES||TritonX||none||none||none||1||0||1||0||TCEP||
 +
|-
 +
| 48||7.5 HEPES||TritonX||Arg||low||PEG||0||0||0||1||TCEP||
 +
|-
 +
| 49||7.5 HEPES||TritonX||Arg||low||Glycerol||1||1||1||0||none||
 +
|-
 +
| 50||7.5 HEPES||TritonX||Glut||high||PEG||1||1||0||0||TCEP||
 +
|-
 +
| 51||7.5 HEPES||Tween80||none||none||Glycerol||1||0||1||1||none||
 +
|-
 +
| 52||7.5 HEPES||Tween80||none||low||PEG||0||1||1||1||DTT||
 +
|-
 +
| 53||7.5 HEPES||Tween80||none||low||PEG||1||1||1||1||TCEP||
 +
|-
 +
| 54||7.5 HEPES||Tween80||Glut||none||none||1||1||1||1||DTT||
 +
|-
 +
| 55||7.5 HEPES||Tween80||Glut||high||none||1||0||0||1||DTT||
 +
|-
 +
| 56||7.5 HEPES||CHAPS||none||low||Glycerol||0||1||0||0||DTT||
 +
|-
 +
| 57||7.5 HEPES||CHAPS||Arg||none||Glycerol||1||1||0||0||TCEP||
 +
|-
 +
| 58||7.5 HEPES||CHAPS||Arg||low||none||0||0||1||1||none||
 +
|-
 +
| 59||7.5 HEPES||CHAPS||Glut||high||PEG||1||0||1||0||DTT||
 +
|-
 +
| 60||8.2 Tris||none||none||high||PEG||0||0||1||1||TCEP||
 +
|-
 +
| 61||8.2 Tris||none||Arg||none||none||1||1||1||0||DTT||
 +
|-
 +
| 62||8.2 Tris||none||Glut||none||none||0||0||1||1||none||
 +
|-
 +
| 63||8.2 Tris||none||Glut||low||none||0||1||0||0||TCEP||
 +
|-
 +
| 64||8.2 Tris||none||Glut||low||Glycerol||1||1||1||0||DTT||
 +
|-
 +
| 65||8.2 Tris||TritonX||none||low||PEG||1||1||1||1||none||
 +
|-
 +
| 66||8.2 Tris||TritonX||none||high||Glycerol||1||0||1||0||none||
 +
|-
 +
| 67||8.2 Tris||TritonX||Arg||high||PEG||1||1||0||1||DTT||
 +
|-
 +
| 68||8.2 Tris||TritonX||Glut||high||PEG||0||1||1||1||TCEP||
 +
|-
 +
| 69||8.2 Tris||Tween80||none||high||none||1||1||0||0||none||
 +
|-
 +
| 70||8.2 Tris||Tween80||Arg||low||Glycerol||0||1||0||0||DTT||
 +
|-
 +
| 71||8.2 Tris||Tween80||Arg||high||Glycerol||1||0||0||1||TCEP||
 +
|-
 +
| 72||8.2 Tris||Tween80||Glut||none||Glycerol||0||0||0||1||none||
 +
|-
 +
| 73||8.2 Tris||CHAPS||none||low||none||1||0||0||0||none||
 +
|-
 +
| 74||8.2 Tris||CHAPS||none||low||Glycerol||0||0||1||1||TCEP||
 +
|-
 +
| 75||8.2 Tris||CHAPS||Arg||none||none||0||1||0||0||DTT||
 +
|-
 +
| 76||8.2 Tris||CHAPS||Arg||none||PEG||1||0||0||1||DTT||
 +
|-
 +
| 77||8.2 Tris||CHAPS||Glut||none||PEG||0||0||1||0||TCEP||
 +
|-
 +
| 78||9.2 CHES||none||none||none||Glycerol||1||1||0||1||TCEP||
 +
|-
 +
| 79||9.2 CHES||none||Arg||none||PEG||0||1||1||0||none||
 +
|-
 +
| 80||9.2 CHES||none||Arg||low||none||1||1||0||1||TCEP||
 +
|-
 +
| 81||9.2 CHES||none||Glut||high||Glycerol||1||0||0||1||DTT||
 +
|-
 +
| 82||9.2 CHES||TritonX||none||none||none||0||0||0||0||TCEP||
 +
|-
 +
| 83||9.2 CHES||TritonX||none||none||Glycerol||0||1||1||1||DTT||
 +
|-
 +
| 84||9.2 CHES||TritonX||none||high||PEG||1||0||1||0||DTT||
 +
|-
 +
| 85||9.2 CHES||TritonX||Arg||low||none||1||1||1||1||DTT||
 +
|-
 +
| 86||9.2 CHES||TritonX||Glut||low||none||0||0||0||0||none||
 +
|-
 +
| 87||9.2 CHES||Tween80||none||high||PEG||0||1||0||0||none||
 +
|-
 +
| 88||9.2 CHES||Tween80||Arg||low||none||1||0||1||0||TCEP||
 +
|-
 +
| 89||9.2 CHES||Tween80||Arg||low||Glycerol||0||0||0||0||none||
 +
|-
 +
| 90||9.2 CHES||Tween80||Glut||none||PEG||0||1||0||0||none||
 +
|-
 +
| 91||9.2 CHES||Tween80||Glut||high||none||1||0||1||0||none||
 +
|-
 +
| 92||9.2 CHES||CHAPS||none||high||Glycerol||1||1||0||1||DTT||
 +
|-
 +
| 93||9.2 CHES||CHAPS||Arg||none||PEG||0||0||0||1||DTT||
 +
|-
 +
| 94||9.2 CHES||CHAPS||Glut||low||PEG||1||0||1||0||none||
 +
|-
 +
| 95||9.2 CHES||CHAPS||Glut||low||Glycerol||0||0||1||1||TCEP||
 
|-
 
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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| 96||9.2 CHES||CHAPS||Glut||high||none||1||1||1||1||TCEP||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
 
|-
 
|-
| colspan="2"|
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|  
==Entry title==
+
|}
* Insert content here...
 
  
 
<!-- ## Do not edit below this line unless you know what you are doing. ## -->
 
|}
 
  
 
==matrix==
 
==matrix==

Revision as of 17:39, 13 October 2009

Buffer # pH detergent amino acids salt crowders divalent cation GnCl BSA Ligand reducing agent Y
1 5.5 MES none none low PEG 1 0 1 0 DTT
2 5.5 MES none none high none 0 0 0 0 DTT
3 5.5 MES none none high PEG 0 0 0 1 none
4 5.5 MES none Arg none PEG 1 0 0 1 TCEP
5 5.5 MES none Glut low Glycerol 1 0 1 0 TCEP
6 5.5 MES TritonX Arg none Glycerol 1 0 1 1 none
7 5.5 MES TritonX Arg high none 0 0 1 0 TCEP
8 5.5 MES TritonX Glut none PEG 0 0 1 0 DTT
9 5.5 MES TritonX Glut low none 0 1 0 1 DTT
10 5.5 MES TritonX Glut low Glycerol 0 1 0 1 none
11 5.5 MES Tween80 none low Glycerol 1 1 1 0 TCEP
12 5.5 MES Tween80 Arg low none 0 0 0 0 DTT
13 5.5 MES Tween80 Arg high Glycerol 0 1 1 1 TCEP
14 5.5 MES Tween80 Glut none Glycerol 1 1 0 1 TCEP
15 5.5 MES Tween80 Glut high PEG 0 1 1 1 none
16 5.5 MES CHAPS none low none 0 1 0 0 none
17 5.5 MES CHAPS none high none 0 1 1 1 TCEP
18 5.5 MES CHAPS none high none 1 1 0 1 DTT
19 5.5 MES CHAPS Arg none PEG 1 0 0 0 none
20 5.5 MES CHAPS Glut none Glycerol 1 1 1 0 DTT
21 6.5 MES none none none none 1 0 0 1 none
22 6.5 MES none none none Glycerol 0 1 1 0 DTT
23 6.5 MES none Arg low none 1 1 1 1 none
24 6.5 MES none Arg high PEG 1 1 1 0 none
25 6.5 MES none Glut low Glycerol 1 0 1 1 DTT
26 6.5 MES TritonX none none Glycerol 1 0 0 0 TCEP
27 6.5 MES TritonX Arg low PEG 0 1 1 1 DTT
28 6.5 MES TritonX Arg high Glycerol 0 0 0 0 DTT
29 6.5 MES TritonX Glut low none 1 1 0 0 TCEP
30 6.5 MES TritonX Glut high Glycerol 1 1 0 1 none
31 6.5 MES Tween80 none none none 0 1 1 1 DTT
32 6.5 MES Tween80 none low PEG 1 0 0 0 TCEP
33 6.5 MES Tween80 none low PEG 1 0 0 1 DTT
34 6.5 MES Tween80 Arg none PEG 0 1 1 0 TCEP
35 6.5 MES Tween80 Glut high none 0 0 1 0 DTT
36 6.5 MES CHAPS none high Glycerol 0 1 1 0 TCEP
37 6.5 MES CHAPS Arg high PEG 1 1 1 0 none
38 6.5 MES CHAPS Arg high Glycerol 0 0 1 1 none
39 6.5 MES CHAPS Glut none none 1 1 0 1 none
40 6.5 MES CHAPS Glut low PEG 0 0 0 1 TCEP
41 7.5 HEPES none none low PEG 0 1 0 1 none
42 7.5 HEPES none Arg high none 0 0 1 1 TCEP
43 7.5 HEPES none Arg high Glycerol 0 1 0 0 none
44 7.5 HEPES none Glut none PEG 0 1 0 0 TCEP
45 7.5 HEPES none Glut high Glycerol 0 0 0 0 DTT
46 7.5 HEPES TritonX none none none 0 0 1 1 none
47 7.5 HEPES TritonX none none none 1 0 1 0 TCEP
48 7.5 HEPES TritonX Arg low PEG 0 0 0 1 TCEP
49 7.5 HEPES TritonX Arg low Glycerol 1 1 1 0 none
50 7.5 HEPES TritonX Glut high PEG 1 1 0 0 TCEP
51 7.5 HEPES Tween80 none none Glycerol 1 0 1 1 none
52 7.5 HEPES Tween80 none low PEG 0 1 1 1 DTT
53 7.5 HEPES Tween80 none low PEG 1 1 1 1 TCEP
54 7.5 HEPES Tween80 Glut none none 1 1 1 1 DTT
55 7.5 HEPES Tween80 Glut high none 1 0 0 1 DTT
56 7.5 HEPES CHAPS none low Glycerol 0 1 0 0 DTT
57 7.5 HEPES CHAPS Arg none Glycerol 1 1 0 0 TCEP
58 7.5 HEPES CHAPS Arg low none 0 0 1 1 none
59 7.5 HEPES CHAPS Glut high PEG 1 0 1 0 DTT
60 8.2 Tris none none high PEG 0 0 1 1 TCEP
61 8.2 Tris none Arg none none 1 1 1 0 DTT
62 8.2 Tris none Glut none none 0 0 1 1 none
63 8.2 Tris none Glut low none 0 1 0 0 TCEP
64 8.2 Tris none Glut low Glycerol 1 1 1 0 DTT
65 8.2 Tris TritonX none low PEG 1 1 1 1 none
66 8.2 Tris TritonX none high Glycerol 1 0 1 0 none
67 8.2 Tris TritonX Arg high PEG 1 1 0 1 DTT
68 8.2 Tris TritonX Glut high PEG 0 1 1 1 TCEP
69 8.2 Tris Tween80 none high none 1 1 0 0 none
70 8.2 Tris Tween80 Arg low Glycerol 0 1 0 0 DTT
71 8.2 Tris Tween80 Arg high Glycerol 1 0 0 1 TCEP
72 8.2 Tris Tween80 Glut none Glycerol 0 0 0 1 none
73 8.2 Tris CHAPS none low none 1 0 0 0 none
74 8.2 Tris CHAPS none low Glycerol 0 0 1 1 TCEP
75 8.2 Tris CHAPS Arg none none 0 1 0 0 DTT
76 8.2 Tris CHAPS Arg none PEG 1 0 0 1 DTT
77 8.2 Tris CHAPS Glut none PEG 0 0 1 0 TCEP
78 9.2 CHES none none none Glycerol 1 1 0 1 TCEP
79 9.2 CHES none Arg none PEG 0 1 1 0 none
80 9.2 CHES none Arg low none 1 1 0 1 TCEP
81 9.2 CHES none Glut high Glycerol 1 0 0 1 DTT
82 9.2 CHES TritonX none none none 0 0 0 0 TCEP
83 9.2 CHES TritonX none none Glycerol 0 1 1 1 DTT
84 9.2 CHES TritonX none high PEG 1 0 1 0 DTT
85 9.2 CHES TritonX Arg low none 1 1 1 1 DTT
86 9.2 CHES TritonX Glut low none 0 0 0 0 none
87 9.2 CHES Tween80 none high PEG 0 1 0 0 none
88 9.2 CHES Tween80 Arg low none 1 0 1 0 TCEP
89 9.2 CHES Tween80 Arg low Glycerol 0 0 0 0 none
90 9.2 CHES Tween80 Glut none PEG 0 1 0 0 none
91 9.2 CHES Tween80 Glut high none 1 0 1 0 none
92 9.2 CHES CHAPS none high Glycerol 1 1 0 1 DTT
93 9.2 CHES CHAPS Arg none PEG 0 0 0 1 DTT
94 9.2 CHES CHAPS Glut low PEG 1 0 1 0 none
95 9.2 CHES CHAPS Glut low Glycerol 0 0 1 1 TCEP
96 9.2 CHES CHAPS Glut high none 1 1 1 1 TCEP


matrix