User:Tara K. Luckau/Notebook/Team ConGen/Entry Base: Difference between revisions

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===PCR===
===PCR===
* comments
* [[Image:20130110_PCRa.png|800 px]]
* [[Image:20130110_PCRa.png|800 px]]


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[[Image:20130110_Gela.png|800 px]]
[[Image:20130110_Gela.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* mispriming everywhere - primer pair not usable for CNHY
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* UAGC Submission# ????
:: [[Image:20130227_Frag.png|900 px]]
:: [[Image:20130227_Frag.png|900 px]]


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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151779 801124151779]
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151779 801124151779]
:: [[Image:20130227_FedEx.png|700 px]]
:: [[Image:20130227_FedEx.png|700 px]]
* UAGC Submission# ????




Revision as of 09:11, 23 May 2013

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page


CNTI Acos5

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


File:20130110 Gela.png


  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
  • favorable conditions indicated by


Fragment Analysis Submission

  • UAGC Submission# ????
File:20130227 Frag.png


File:20130227 FedEx.png


  • FedEx picked up ____pm, 26 February
  • FedEx delivered ____am, 27 February
  • UAGC received ____am, 27 February
  • UAGC completed ____am, 28 February




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