Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/Entry Base"

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===PCR===
 
===PCR===
* [[Image:20130110_PCRa.png|800 px]]
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 +
 
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* comments
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:: [[Image:20130110_PCRa.png|800 px]]
  
  
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[[Image:20130110_Gela.png|800 px]]
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:: [[Image:20130110_Gela.png|800 px]]
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* mispriming everywhere - primer pair not usable for CNHY
 
* mispriming everywhere - primer pair not usable for CNHY
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:: [[Image:20130226_Frag.png|900 px]]
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* UAGC Submission# ????
 
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:: [[Image:20130227_Frag.png|900 px]]
 
 
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151778 801124151778]
 
:: [[Image:20130226_FedEx.png|700 px]]
 
  
  
* UAGC Submission# PPTX
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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151779 801124151779]
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:: [[Image:20130227_FedEx.png|700 px]]
  
  
* FedEx picked up 2:44pm, 26 February
+
* FedEx picked up ____pm, 26 February
* FedEx delivered 9:29am, 27 February
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* FedEx delivered ____am, 27 February
* UAGC received 10:58pm, 27 February
+
* UAGC received ____am, 27 February
* UAGC completed 10:41am, 28 February
+
* UAGC completed ____am, 28 February
  
  

Revision as of 08:12, 23 May 2013

Owwnotebook icon.png Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page


CNTI Acos5

PCR

  • comments
800 px


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


800 px


  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (Orangefrownyface.png), if needed, under stringent conditions
  • favorable conditions indicated by Greenhappyface.png


Fragment Analysis Submission

  • UAGC Submission# ????
900 px


700 px


  • FedEx picked up ____pm, 26 February
  • FedEx delivered ____am, 27 February
  • UAGC received ____am, 27 February
  • UAGC completed ____am, 28 February