Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/Entry Base"

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==Task==
+
==CNTI Acos5==
* here
 
* here
 
** in here
 
# numbered
 
# again
 
  
  
===Subsection===
+
===PCR===
* here
+
* [[Image:20120110_PCRa.png|800 px]]
 +
 
 +
 
 +
===Gel===
 +
 
 +
{| {{table}} style="text-align: center"
 +
|-
 +
! Pour !! Load !! Run
 +
|-
 +
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 +
|-
 +
| 27 µL GelRed || 6 µL ladder || 45 minutes
 +
|}
 +
 
 +
 
 +
[[Image:20120110_Gela.png|800 px]]
 +
 
 +
* mispriming everywhere - primer pair not usable for CNHY
 +
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 +
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 +
 
 +
 
 +
===Fragment Analysis Submission===
 +
 
 +
 
 +
:: [[Image:20130226_Frag.png|900 px]]
 +
 
 +
 
 +
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151778 801124151778]
 +
:: [[Image:20130226_FedEx.png|700 px]]
 +
 
 +
 
 +
* UAGC Submission# PPTX
 +
 
 +
 
 +
* FedEx picked up 2:44pm, 26 February
 +
* FedEx delivered 9:29am, 27 February
 +
* UAGC received 10:58pm, 27 February
 +
* UAGC completed 10:41am, 28 February
 +
 
  
  

Revision as of 15:02, 12 March 2013

Owwnotebook icon.png Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page


CNTI Acos5

PCR

  • 20120110 PCRa.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20120110 Gela.png

  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (Orangefrownyface.png), if needed, under stringent conditions
  • favorable conditions indicated by Greenhappyface.png


Fragment Analysis Submission

20130226 Frag.png


20130226 FedEx.png


  • UAGC Submission# PPTX


  • FedEx picked up 2:44pm, 26 February
  • FedEx delivered 9:29am, 27 February
  • UAGC received 10:58pm, 27 February
  • UAGC completed 10:41am, 28 February